| 人乳头瘤病毒HPV16E6的表达促进角质生成细胞凋亡的发生 |
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| 引用本文： | 刚毅,王红红,赵丽娜,李颖钊,刘志国. 人乳头瘤病毒HPV16E6的表达促进角质生成细胞凋亡的发生[J]. 科学技术与工程, 2009, 9(15) |
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| 作者姓名： | 刚毅 王红红 赵丽娜 李颖钊 刘志国 |
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| 作者单位： | 第四军医大学西京消化病院肿瘤生物学国家重点实验室,西安,710032;第四军医大学西京消化病院肿瘤生物学国家重点实验室,西安,710032;第四军医大学西京消化病院肿瘤生物学国家重点实验室,西安,710032;第四军医大学西京消化病院肿瘤生物学国家重点实验室,西安,710032;第四军医大学西京消化病院肿瘤生物学国家重点实验室,西安,710032 |
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| 基金项目： | 国家自然科学基金(30400016)项目 |
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| 摘 要： | 为探讨HPV16早期基因E6的表达对角质生成细胞NIKS凋亡表型的影响及可能的机制,经培养稳定表达HPV16E6的角质生成细胞NIKS;使用不同的DNA损伤剂处理转染后细胞,流式细胞术检测细胞凋亡情况;建立表达HPV16E6突变体Y54D及F2V的p53降解缺陷型NIKS细胞系并用DNA损伤剂处理,流式细胞术检测凋亡情况.研究显示,流式细胞术结果显示稳定表达E6的细胞系在足叶乙甙、丝裂霉素及紫衫醇处理后发生明显的细胞凋亡;表达Y54D或F2V的NIKS细胞经DNA损伤剂处理后凋亡明显低于正常E6表达细胞.研究表明,人乳头瘤病毒E6的表达能够促进角质生成细胞凋亡的发生,这种促凋亡作用与细胞内p53的表达相关.
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| 关 键 词： | HPV16 E6 角质生成细胞 凋亡 p53 |
Expression of Human Papillomavirus 16E6 Gene in Keratinocytes Promote Apoptosis Induced by DNA Damage |
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| GANG Yi,WANG Hong-hong,ZHAO Li-na,LI Ying-zhao,LIU Zhi-guo. Expression of Human Papillomavirus 16E6 Gene in Keratinocytes Promote Apoptosis Induced by DNA Damage[J]. Science Technology and Engineering, 2009, 9(15) |
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| Authors: | GANG Yi WANG Hong-hong ZHAO Li-na LI Ying-zhao LIU Zhi-guo |
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| Affiliation: | State Key Laboratory of Cancer Biology & Xijing Hospital of Digestive Diseases;Fourth Military Medical University;Xi'an;710032;P.R.China |
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| Abstract: | To investigate the effect of Human papillomavirus type 16E6 on apoptosis of NIKS induced by DNA damaging agents and possible mechanism. Human keratinocyte NIKS cell line stably expressing HPV16E6 was cultured. Then cells were treated with common DNA damaging reagents including Etoposide,cisplatin,Mitomycin and taxel. The apoptosis of cells was assessed by flow cytometry. Cell line expressing HPV16E6 mutants Y54D and F2V was constructed,respectively. Then the cells were treated with DNA damaging agent and as... |
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| Keywords: | HPV16 E6 p53 |
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