饥饿对两种织纹螺肉体化学组分的影响

目的研究饥饿对两种海洋食腐动物(Nassarius siquijorensis和Nassarius conoidalis)肉体化学组分的影响。方法采用标准生化测定法研究了饥饿状态下这两种织纹螺肉体组织中营养成分组成:粗脂肪的测定采用索氏抽提法;粗灰分的测定采用高温灰化法;凯氏定氮法测定粗蛋白质。结果经过32天的饥饿处理,发现两种织纹螺肉体的粗蛋白质和粗脂肪含量显著降低,碳水化合物含量显著升高(P<0.05)。结论研究结果表明当食物缺乏的时候,N.conoidalis和N.Siquijorensis会通过调动像蛋白质和脂肪这样的内源性生理学燃料,来满足其进行基本生命活动所需的能量。

Effects of hunger on somatic chemical composition of two subtidal nassariid gastropods, Nassarius siquijorensis and Nassarius conoidalis

Objective-To study the effects of hunger on somatic chemical composition of marine scavengers which play an important ecological role on the sea bed. Methods-Crude fat was extracted following the method of Bligh and Dyer （1959） and Chan et al. （2004）. Carbon and nitrogen contents were determined with the catalyzed combustion method. Protein content was obtained with the tradi-tion Protein-nitrogen conversion factor of 6.9,5 （Allen 1989）. Inorganic residue was obtained by using the Dry ashing method （Leniel H. Harbers）. Results-After 32 days of starvation, significantly lower percentage of protein and fat but higher percentage of carbohydrate were found in the unfed group as compared with the control for both N. siquijorensis and N. conoidalis. Conclusion-When food was deprived, N. conoidalis and N. Siquijorensis mobilized endogenous physiological fuels such as pro-teins, lipids, and carbohydrates to meet the energy required to carry out the basic processes of life.