镁合金基HA材料生物相容性及生物活性的研究

采用电泳沉积法制备了镁合金基羟基磷灰石表面涂层生物复合材料.利用动物体内埋植实验和SBF浸泡实验分别对复合材料生物相容性及生物活性进行研究.将SD大鼠分为对照组和植入组,植入一定的时间后,取植入物周围组织进行切片、HE染色和组织分析;将复合材料恒温浸泡于SBF溶液四周后取出,通过检测复合材料表面生长磷灰石的能力作为评价该材料的生物活性.实验结果是植入物周围出现组织增生,有结缔组织及新生血管的形成,未见明显的组织炎症反应,无多核的巨细胞、淋巴细胞和中性粒细胞等炎细胞的浸润;复合材料浸泡于SBF溶液后,复合材料表面形成一层类骨磷灰石.表明镁合金基羟基磷灰石生物复合材料无细胞毒性,不引起机体免疫反应,能诱导磷灰石的形成,具有良好的生物相容性及生物活性.

Biocompatibility and Bioactivity of MagnesiumMatrix Hydroxyapatite Material

Magnesium matrix hydroxyapatite biological composite material was prepared by electrophoretic deposition method,and the biocompatibility and biological activity of this material was discussed.Animal experiment and SBF immersion experiment were selected separately to test the biocompatibility and bioactivity.The SD rats were divided into contrast group and implant group.The implant material surrounding tissue was taken to do Tissue biopsy,HE dyed and organizational analysis after a certain amount of time in the SD body.Under homeothermic condition,the biological composite material was soaked in SBF solution.After four weeks,the bioactivity of the biological composite material was evaluated by testing the growth ability of hydroxyapatite on composite material.The experiment results showed that the implant material surrounding appeared tissue hyperplasia,connective tissue and new blood vessels,but no obvious tissue inflammation,no infiltrating inflammatory cells such as multinuclear giant cells and neutrophils cells.After the biological composite material was soaked in SBF solution,a layer Bone-Like Apatite was found on its surface.The results showed that Magnesium matrix hydroxyapatite biological composite material have no cytotoxicity,induction bone formation,good biocompatibility and bioactivity.