RAPD技术在实验动物中的应用

随着生物医学的发展 ,实验动物种质资源的保护和利用、实验动物的标准化问题已成为当今实验动物科学发展的主题。实验动物遗传背景分析、品种或品系的鉴定、引种和繁育均需要一定的遗传标记辅助完成 ,分子遗传标记作为一种有效精确的监测手段 ,突破了形态学标记、细胞学标记和同功酶标记等表达型标记的局限性 ,可以从分子水平揭示物种的遗传变异 ,在许多领域发挥着独特的作用。RAPD技术是 1990年被首次提出 ,由于该方法具有简便、快速、成本低、信息含量丰富等特点 ,在生物类群种属分类鉴定、遗传图谱构建、物种亲缘关系的确定以及种群遗…

Application of RAPD Markers in Laboratory Animal

With the sharp development of biomedicine, the protection and utilization of laboratory animal resources, and standardization of laboratory animal have been regarded as the theme of laboratory animal science. Many kinds of genetic markers were used in genetic background analysis, identification of species and strains, and breeding of laboratory animal, unlike morphological marker, cytological marker and isozyme marker, which revealed the difference at gene expressing level, molecular genetic markers used as an accurate and effective method reveal genetic variations of hereditary material, it plays an important role in many fields. The RAPD (Random Amplification Polymorphic DNA) method was firstly described in 1990, it has several distinct advantages over other techniques in that it is easy to perform and fast, and so was widely applied to taxonomy and identification of species, construction of genetic mapping, genetic relationships among species and population genetics. This paper summarized the application of RAPD markers in laboratory animal in recent 10 years.