| 不同纬度野生大豆种群间的遗传变异 |
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| 引用本文： | 钱吉 陈痒. 不同纬度野生大豆种群间的遗传变异[J]. 复旦学报(自然科学版), 1998, 37(2): 208-212 |
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| 作者姓名： | 钱吉 陈痒 |
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| 摘 要： | 选用了经初步筛选的２０个短随机序列引物和２个长随机序列引物对５个不同纯度来源的野生大豆进行了ＤＮＡ随机扩增，其中有１９个引物扩增出多态性片段，区获得７０个ＲＡＰＤ多态性片段，平均每个引物３．６８条，运用自编的计算机程序对扩增进行聚类分析。
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| 关 键 词： | 野生大豆 大豆 种群间遗传 生态学 变异 |
Genetic Variations Among Populations of Wild Soybeans from Different Latitude |
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| Qian Ji, Chen Xiang, Zheng Shizhang, Chen Ran. Genetic Variations Among Populations of Wild Soybeans from Different Latitude[J]. Journal of Fudan University(Natural Science), 1998, 37(2): 208-212 |
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| Authors: | Qian Ji Chen Xiang Zheng Shizhang Chen Ran |
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| Affiliation: | Department of Environment and Resource Biology |
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| Abstract: | Random amplified polymorphic DNA (RAPD) analysis was applied to five populations of wild soybeans (Glycine soja ) originated from different latitudes. Altogether twenty screened random 10-mer primers and two random 20-mer primers were used to assay polymorphisms among populations. Nineteen primers including one 20-mer primer gave polymophic products and yielded seventy RAPD bands. Bands were scored (+/- ) and unweighted pair group method with arithmetic mean (UPGMA) was used to generate dendrogrmas showing genetic relationships among the G. soja populations evaluated. Cluster analysis showed there were some genetic variations among the G. soja populations and some noticeable correlations existed between the variations and the latitude. |
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| Keywords: | Glycine soja RAPD PCR UPGMA |
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