Calcium release from frog sarcoplasmic reticulum by an imidazolyl reagent |
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| Authors: | T. Aoki T. Oba |
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| Affiliation: | (1) Department of Physiology, Nagoya City University Medical School, Mizuho-ku, 467 Nagoya, (Japan);(2) Department of Food and Nutrition Science, Gifu City Women's Junior College, 2693 Nagarafukumitsu, 502 Gifu, (Japan) |
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| Abstract: | Summary Calcium is released from the isolated heavy sarcoplasmic reticulum (SR) of frog skeletal muscle upon application of 0.1–1 mM diethylpyrocarbonate (DEP, an imidazolyl reagent). The Ca-ATPase activity of SR was suppressed by 20% in the presence of 1 mM DEP. More than 1 mM of free magnesium ion or 5  M ruthenium red eliminated the effect of DEP on calcium release but not on Ca-ATPase activity. A plausible site of DEP action is on the calcium channel. |
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| Keywords: | Fragmented sarcoplasmic reticulum diethylpyrocarbonate calcium release Ca-ATPase |
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