甜地丁总黄酮提取工艺及体外抗氧化活性研究

为研究甜地丁总黄酮提取工艺及体外抗氧化活性，本文利用L9（34）正交试验，探讨了提取条件对总黄酮提取量的影响，并采用Fenton体系和邻苯三酚自氧化法评价了该提取物的体外抗氧化能力，采用多元线性回归对总黄酮与抗氧化活性相关性进行了分析。结果显示最佳提取条件为20倍量75%乙醇，提取4次，提取时间为30 min，此条件下的黄酮得率为3.423%；甜地丁总黄酮对·OH和O2-·清除能力与浓度呈较明显的量效关系，且抗氧化作用与总黄酮量之间正相关。甜地丁总黄酮具有较强的抗氧化能力，是一种天然有效的抗氧化剂。

Optimal extraction and antioxidative activity of total flavonoids from Herba Gueldenstaedtiae

We addressed the impact of extraction conditions on the extraction amount of total flavonoids with L9 （34） orthogonal test.We also evaluated its antioxidative activity by Fenton system and pyrogallol autooxidation method.We further analyzed the correlation between total flavonoids and antioxidative activity with multiple linear regression.Results show that the optimal extraction conditions are 75％ ethanol （solid and liquid ratio of 1 ∶ 20）,4 times extraction and extraction time of 30 min.Its optimal extraction rate is 3.423％.Obvious dose-effect relationship is shown between concentration and its clearance capability to · OH and O2-·.Positive correlation is also exhibited between antioxidative activity and total flavonoids amount.Total flavonoids from Herba Gueldenstaedtiae have stronger antioxidative activity and are effective natural antioxidants.