鄱阳湖南矶湿地苔草植被根际土壤微生物群落结构分析

为探究鄱阳湖南矶湿地苔草植被根际土壤微生物群落结构，该文以鄱阳湖南矶湿地苔草植被根际土壤为研究对象，采取0～15 cm、15～30 cm及30～45 cm深度的土壤，采用Illumina HiSeq高通量测序技术，研究不同深度土壤群落结构及其影响因素.研究结果表明：不同深度土壤样品理化性质和酶活性存在显著差异，随土壤深度的增加呈降低趋势.Pearson相关分析表明鄱阳湖南矶湿地苔草植被根际土壤理化性质与土壤酶活性之间存在不同程度的相关性.高通量测序结果表明不同深度土壤细菌和真菌群落微生物丰富度及多样性未达到在统计学意义上的差异显著水平(P>0.05),土壤古菌群落微生物多样性随土壤深度的增加而增加.在苔草植被根际土壤中共检测到41个细菌门类，其中变形菌门、酸杆菌门、绿弯菌门和硝化螺旋菌门为优势菌门；检测到8个真菌门类，其中子囊菌门、担子菌门和罗兹菌门为优势菌门；检测到10个古菌门类，其中广古菌门、泉古菌门、奇古菌门为优势菌门.冗余分析表明：土壤有机质、全氮、全磷和碱解氮含量是影响细菌群落的主要环境因子；土壤有机质、全氮、有效磷和碱解氮含量是影响真菌群落的主要环境因子；土壤有机质、...

The Analysis of Microbial Community Structure in Rhizosphere Soil of Carex Vegetation in Poyang Lake Nanji Wetland

In order to explore the effects of microbial community structure in rhizosphere soil of Carex vegetation in Poyang Lake Nanji Wetland,Illumina HiSeq high-throughput sequencing technology is applied to examine the soil community structure and its influencing factors at the layer of 0—15,15—30 and 30—45 cm of Carex vegetation soil in Nanji Wetland.The results show that there are significant differences in the physical and chemical properties and enzyme activities of soil samples at different depths.Pearson correlation analysis shows that there are varying degrees of correlation between them.High-throughput sequencing results show that the microbial richness and diversity of soil bacterial and fungal communities at different depths did'n reach significant differences(P>0.05)and archaeal soil microbial diversity increases with soil depth.A total of 41 bacterial phyla,8 fungal phyla and 10 archaea phyla are obtained from different treatments,of which the dominant bacterial phyla are Proteobacteria,Acidobacteria,Chloroflexi and Nitrospirae,the dominant fungal phyla are Ascomycota,Basidiomycota and Mortierellomycota,the dominant archaea phyla are Euryarchaeota,Crenarchaeota and Thaumarchaeota.The redundancy analysis shows that organic matter,total nitrogen,total phosphorus and alkaline hydrolyzed nitrogen have greater impact on bacterial community structure,while organic matter,total nitrogen,available phosphorus and alkali hydrolyzable nitrogen content have greater impact on fungal community structure.And organic matter,total nitrogen,total phosphorus and total potassium content have greater impact on archaea community structure.