DSA 联合 CT 引导下经皮肝穿刺兔 VX2 肝癌模型制作

摘要: 目的 探讨 DSA 联合 CT 引导下经皮肝穿刺兔 VX2 肝癌模型制作的可行性。方法 40 只新西兰白兔随机分 成 A、B 两组，每组 20 只，A 组在 DSA 联合 CT 引导下将兔 VX2 肿瘤组织块经皮肝穿刺接种于兔左肝内，B 组通过 开腹法种植 VX2 肿瘤组织块，于接种后第 2 周、4 周行全身 MR 检查，后处死实验兔行原位、异位肿瘤肉眼观察。结 果 A 组建模成功率 90% ，有 1 只出现异位种植，未发现腹水及死亡，平均手术时间( 17. 3 ± 5. 2) min; B 组建模成 功率 85% ，有 3 只出现腹水，2 只死亡，无异位种植发生，平均手术时间( 50. 7 ± 11. 3) min，，两组手术时间比较差异 有统计学意义( P ＜ 0. 05) 。结论 DSA 联合 CT 引导下经皮肝穿刺制作兔 VX2 肝癌模型方法简单、建模成功率高、 手术操作时间短。

Establishment of Rabbit liver VX2 Tumor Model via Percutaneous Transhepatic Approach under DSA and CT Guidance

Abstract: Objective To evaluate the establishment method of rabbit liver VX2 tumor model by injecting tumor tissue mass into the liver parenchyma via percutaneous transhepatic approach under DSA and CT guidance． Method Forty New Zealand rabbits were divided into A and B groups randomly with 20 rabbits each． The VX2 tumor tissue mass was implanted into rabbits liver guided through percutaneous transhepatic route guided by DSA and CT in group A and through epigastrium incision method in group B． On the second and third weeks after implantation， MR examination was performed and the rabbits were sacrificed to observe the tumor in situ and ectopia． Result In group A，the success rate of the liver VX2 tumor model was 90% with mean operation time of ( 17. 3 ± 5. 2) min and there was no episode of ascites and death with the exception of ectopia implantation in one case． In group B， successful establishment rate was 85% with average operation time as long as ( 17. 3 ± 5. 2) min and ascites and death were observed in three and two cases respectively． The difference of operation time between two groups was statistically significant ( P ＜ 0. 05) ． Conclusion The method of liver VX2 tumor implantation guided by DSA and CT via percutaneous transhepatic approach is simple with higher success rate and shorter operation time for making liver carcinoma model of rabbits．