玉米自交系在缺磷胁迫下的5种抗氧化酶活性变化的研究

以珍珠岩作基质固体培养4种不同基因型玉米自交系材料，设置：0．25mol／LKH2PO4全磷（对照）、0mol／LKH2PO4（缺磷）2种处理，于第5，7，9，11d考察超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）、谷胱甘肽还原酶（GR）活性的变化．缺磷时不同自交系相同抗氧化酶活性的变化趋势不一致．相同自交系的不同抗氧化酶在缺磷胁迫下的变化趋势也有差异．结论：不能仅用1种膜保护酶在胁迫时活性的高低来判断材料是耐缺磷胁迫型还是缺磷敏感型．应综合考虑这5种保护酶活性．

Study on Five Different Antioxidant Activities of Maize Inbred Lines Under Phosphorus Deficiency Stress

Under solid cultivating with perlite substrate,four maize inbred lines were subjected to 2 phosphorus deficiency levels: 1) Full P level(CK) with 0.25 mol/L KH2PO4,2) No P level(NP).The SOD activity,POD activity,CAT activity,APX activity and GR activity were investigated on the 5th,7th,9th,11th day.Under no phosphorus condition,not only the activities of the same antioxidase in the different lines showed the different trends of changes,but also the activities of the different antioxidase in the same lines.The results indicated that it was unreliable to adopt only one protective enzyme to judge whether a maize inbred line was tolerant or sensitive to low phosphorus condition.Accurate evaluation can only be made based on a series of indices,such as the activities of superoxide dismutase(SOD),peroxide dismutase(POD),catalase(CAT),ascorbate peroxidase(APX) and glutathione reductase(GR).