| 两种蚂蚁体内wolbachia的WSP基因序列测定 |
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| 引用本文： | 邓素芳,陈长缨,许升全.两种蚂蚁体内wolbachia的WSP基因序列测定[J].陕西师范大学学报,2006,34(Z1):49-51. |
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| 作者姓名： | 邓素芳 陈长缨 许升全 |
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| 作者单位： | 陕西师范大学动物研究所 陕西西安710062(邓素芳，许升全)，新疆克拉玛依市第九中学 新疆克拉玛依834003(陈长缨) |
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| 摘 要： | 应用WSP基因通用引物,通过PCR方法,对所采日本弓背蚁和棒刺大头蚁体内的 wolbachia进行检测．结果发现在部分蚂蚁个体中可以扩增出约550 bp(日本弓背蚁)和530 bp(棒刺大头蚁)的特异性目的片段．表明所采集的蚂蚁标本中具有沃尔巴克氏体．将所得WSP基因序列与Gen Bank中的蚁科已知wolbachia品系WSP基因序列作比对分析,发现它们同A大组的 InsA组的wolbachia株十分相近．
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| 关 键 词： | 日本弓背蚁 棒刺大头蚁 沃尔巴克氏体(Wolbachia) |
| 文章编号： | 1672-4291(2006)Sup.-0049-03 |
| 修稿时间： | 2005年10月12 |
Sequence analysis of the WSP gene of wolbachia in two ant species |
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| DENG Su-fang,CHE Chang-ying,XU Sheng-quan.Sequence analysis of the WSP gene of wolbachia in two ant species[J].Journal of Shaanxi Normal University: Nat Sci Ed,2006,34(Z1):49-51. |
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| Authors: | DENG Su-fang CHE Chang-ying XU Sheng-quan |
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| Abstract: | Universal primers of the WSP gene sequence were used to amplify DNA of Wolbachia from ants by polymerase chain reaction (PCR). Some 550 bp (Camponotus japonicus Mayr) and 530bp (Pheidole spathifera Forel) characteristic products were observed after amplification of extracts from some specimens. The results show that the ant specimens were infected by wolbachia. By constructing the phylogenetic tree of the WSP gene sequence of Camponotus japonicus Mayr, Pheidole spathifera Forel and that sequences from Gen Bank, we found that the sequences of two ant species were similar to the strain of InsA group in A supergroup of wolbachia. |
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| Keywords: | Camponotus japonicus Mayr Pheidole spathifera Forel wolbachia WSP |
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