| 氨基酸对映体的胶束电动毛细管色谱拆分研究 |
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| 引用本文： | 盛筱,尤进茂.氨基酸对映体的胶束电动毛细管色谱拆分研究[J].曲阜师范大学学报,2009,35(4):71-75. |
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| 作者姓名： | 盛筱 尤进茂 |
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| 作者单位： | [1]济宁医学院药学院,日照市276826 [2]曲阜师范大学化学科学学院,山东省曲阜市273165 |
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| 摘 要： | 以2-（11H-苯a]咔唑）乙基氯甲酸酯（BCEC—Cl）作为柱前衍生试剂对氨基酸进行衍生，以三（羟甲基）氨基甲烷-磷酸（Tris—H3PO4）溶液作为缓冲溶液，β-环糊精（β—CD）为手性拆分试剂，十二烷基硫酸钠（SDS）为表面活性剂，采用胶束电动毛细管色谱模式考察并优化了氨基酸对映体的分离条件．结果实现了15种氨基酸对映体的拆分，结果令人满意．
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| 关 键 词： | BCEC—Cl 氨基酸对映体 手性拆分 胶束电动毛细管色谱 |
Chiral Separations of BCEC- Cl-Derivatized Amino Acids byMicellar Electrokinetic Capillary Chromatography |
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| SHENG Xiao,YOU Jin-mao.Chiral Separations of BCEC- Cl-Derivatized Amino Acids byMicellar Electrokinetic Capillary Chromatography[J].Journal of Qufu Normal University(Natural Science),2009,35(4):71-75. |
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| Authors: | SHENG Xiao YOU Jin-mao |
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| Institution: | SHENG Xiao, YOU Jin-mao(1. Pharmaceutical College, Jining Medical University, 276826,Rizhao;2.College of Chemistry Science, Qufu Normal University, 273165,Qufu, Shandong,PRC) |
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| Abstract: | A method for the chiral separations of amino acid enantiomers by micellar electrokinetic capillary chromatography has been proposed , with 2- （ 11 H - benzo a ] carbazol-11-yl） ethyl earbonochloridate （ BCEC - Cl） as derivatization reagent , tris（hydroxymethyl） aminomethane-phosphoric acid solution （Tris -H3PO4 ） as buffer so- lution , β-Cyclodextrin （β -CD） as chiral selector, and Sodium Dodecyl Sulfate （SDS） as surfactant, under the optimized conditions , 15 pairs of amino acid enantiomers have been completely resolved , the results are satisfactory. |
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| Keywords: | BCEC-Cl |
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