| Crocin Synthesis Mechanism in Crocus sativus |
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| 作者姓名： | 杨波 郭志刚 刘瑞芝 |
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| 作者单位： | Department of Chemical Engineering, Tsinghua University, Beijing 100084, China |
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| 摘 要： | Saffron （Crocus sativus） cells can synthesize crocin, crocetin digentiobiosyl ester, in suspension cultures. The crocin family biosynthesis mechanism was studied using high pressure liquid chromatography （HPLC） to determinate the glucosyltransferase activity and to develop a method for synthesizing medicine from saffron cells. Previous studies indicated that two glucosyltransferases might be involved in the formation of crocetin glucosyl- and gentiobiosyl-esters. GTasel formed an ester bond between crocetin carboxyl groups and glucose moieties while GTase2 catalyzed the formation of glucosidic bonds with glucosyl ester groups at both ends of the molecule. These enzymes can catalyze the formation of crocetin glucosides in vitro. GTasel activity is higher during the first four days of crocin glucosides biosynthesis, but decreases after four days. The formation and accumulation of crocin increase during the first six days and stabilized on the eighth day.
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| 关 键 词： | 藏花素 藏红花 生物合成 HPLC 糖基转移酶 |
| 收稿时间： | 2003-11-07 |
| 修稿时间： | 2003-11-07 |
Crocin Synthesis Mechanism in Crocus sativus |
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| Bo Yang, &#x;Î, Zhigang Guo, íý
,Ruizhi Liu, &#x;
.Crocin Synthesis Mechanism in Crocus sativus[J].Tsinghua Science and Technology,2005,10(5):567-572. |
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| Authors: | Bo Yang &#x;Î Zhigang Guo íý
Ruizhi Liu &#x;
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| Institution: | Department of Chemical Engineering, Tsinghua University, Beijing 100084, China |
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| Abstract: | Saffron (Crocus sativus) cells can synthesize crocin, crocetin digentiobiosyl ester, in suspension cultures. The crocin family biosynthesis mechanism was studied using high pressure liquid chromatography (HPLC) to determinate the glucosyltransferase activity and to develop a method for synthesizing medicine from saffron cells. Previous studies indicated that two glucosyltransferases might be involved in the forma- tion of crocetin glucosyl- and gentiobiosyl-esters. GTase1 formed an ester bond between crocetin carboxyl groups and glucose moieties while GTase2 catalyzed the formation of glucosidic bonds with glucosyl ester groups at both ends of the molecule. These enzymes can catalyze the formation of crocetin glucosides in vi- tro. GTase1 activity is higher during the first four days of crocin glucosides biosynthesis, but decreases after four days. The formation and accumulation of crocin increase during the first six days and stabilized on the eighth day. |
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| Keywords: | saffron crocetin crocetin di-neapolitanosyl ester glucosyltransferase glycosylation |
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