黄瓜再生体系的建立

本实验以黄瓜(CucumissativusL.)品种“LS-21”为实验材料,按如下培养基配方:MS培养基,附加6-BA(0.8～1.6)mg/l IAA0.2mg/l;不同浓度的6-BA分别与NAA及2,4-D配合,研究影响黄瓜组织培养的诸多因素,建立了黄瓜离体子叶高频不定芽再生系统。主要结果如下:黄瓜5-6日苗龄无菌苗子叶作外植体,愈伤组织生长旺盛,芽诱导率高;7-8天的外植体则生长势弱,不利再生芽的分化。6-BA(1.2～1.6)mg/l IAA0.2mg/l,芽分化频率较高,其中6-BA1.2mg/l的芽分化率可达51%;待再生芽长至2-3cm时移入生根培养基(1/2MS 0.1mg/LIAA),生根率达90%,40-60d可得到完整再生植株。

Establishing Regeneration Systems on cucumber cotyledons

In this study,high efficiency of shoots regeneration conditions were established,and factors impacting cucumber tissue cultivation were studied by the basic medium MS with different hormones include 0.2 mg/L IAA and 0.8~1.6 mg/L 6-BA(W);6-BA with different concentration of NAA and 2,4-D for callus and shoots induction.The main results are as follows: callus growths well when use 5 day' cotyledons as explants than that of 7~8 days,the same results occurs in shoots regeneration rate.Higher efficiency of shoots regeneration obtained from MS medium added 0.2 mg/L IAA and 1.2~1.6 mg/L 6-BA(W),especially,in MS 1.2 mg/L 6-BA 0.2mg/l IAA,51% regeneration rate appeared,which is the highest rate of shoots regeneration in our experiments.The adventitious shoots were placed on medium(MS 0.1 mg/L IAA) for root regeneration,the rate is 90%.The whole regenerated plants could be obtained in 40-60 days.