| 一株万古霉素产生菌的分离及育种 |
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| 引用本文： | 王明兹,黄翠莲,吴莹莹.一株万古霉素产生菌的分离及育种[J].厦门大学学报(自然科学版),2011,50(5):915-921. |
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| 作者姓名： | 王明兹 黄翠莲 吴莹莹 |
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| 作者单位： | 厦门大学生命科学学院,福建厦门,361005 |
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| 基金项目： | 国家自然科学基金项目(31070053); 福建省教育厅科技项目(JB06092) |
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| 摘 要： | 从厦门集美的土壤样品中分离了一株发酵产物对金黄色葡萄球菌(Staphylococcus aureus)CMCC(B)26003和枯草芽孢杆菌(Bacillus subtilis)CMCC(B)63501具有明显抑菌活性的放线菌菌株(XM0301).经形态、生理、生化鉴定和16S rDNA序列分析,为一株东方拟无枝酸菌(A mycolatopsis orientalis),高效液相色谱(HPLC)检测其发酵产生万古霉素.随后采用原生质体诱变和基因组改组提高其万古霉素的产量,经初筛、复筛获得万古霉素产量达到4 210μg/mL的高产突变株G2-73.
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| 关 键 词： | 东方拟无枝酸菌XM0301 万古霉素 原生质体 诱变 基因组改组 |
A Vancomycin-producing Strain Isolation and Improving Its Production |
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| WANG Ming-zi,HUANG Cui-lian,WU Ying-ying.A Vancomycin-producing Strain Isolation and Improving Its Production[J].Journal of Xiamen University(Natural Science),2011,50(5):915-921. |
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| Authors: | WANG Ming-zi HUANG Cui-lian WU Ying-ying |
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| Institution: | WANG Ming-zi,HUANG Cui-lian,WU Ying-ying (School of Life Sciences,Xiamen University,Xiamen 361005,China) |
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| Abstract: | An actinomycetes strain(XM0301),which showed strong antimicrobial activity on Staphylococcus aureus CMCC(B)26003 and Bacillus subtilis CMCC(B)63501,was isolated from the soil sample from Jimei,Xiamen.By the evidences of morphology and 16S rDNA sequences analysis,it belongs among the strains of Amycolatopsis orientalis.The XM0301 stain slso produces vancomycin,a kind of glycopeptide antibiotics producing by Amycolatopsis orientalis.Protoplast mutation and genome shuffling have been introduced to improve vanc... |
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| Keywords: | Amycolatopsis orientalis XM0301 vancomycin protoplast mutation genome shuffling |
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