| 高效液相色谱法测定嘎日迪-5味丸中绿原酸的含量 |
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| 引用本文： | 苏日塔拉图,席海山,孙乌日娜.高效液相色谱法测定嘎日迪-5味丸中绿原酸的含量[J].内蒙古民族大学学报(自然科学版),2009,24(1):82-84. |
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| 作者姓名： | 苏日塔拉图 席海山 孙乌日娜 |
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| 作者单位： | 1. 内蒙古民族大学附属医院,内蒙古,通辽,028000
2. 内蒙古民族大学,分析测试中心,内蒙古,通辽,028000 |
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| 摘 要： | 目的：建立测定蒙药嘎日迪-5味丸中绿原酸含量的反相高效液相色谱法．方法：用ODS C18色谱柱，流动相为乙腈-水-冰醋酸（11．5：88．5：2），检测波长318nm，流速为1ml／min．以外标法测定了嘎日迪-5味丸中绿原酸的含量．结果：绿原酸在4．5～22．5μg/ml浓度范围内呈良好的线性关系．平均回收率为98．1％，RSD=2．2％（n=5）．结论：本法简便，可靠，可作为蒙药嘎日迪-5味丸的含量测定方法．
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| 关 键 词： | 嘎日迪-5味丸 高效液相色谱法 绿原酸 |
Determination of the Content of Chlorogenic Acid in Mongolian Medicine Garidi-5 Pills by HPLC |
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| Suritalatu,Xi Hai-shan,SUN Wurina.Determination of the Content of Chlorogenic Acid in Mongolian Medicine Garidi-5 Pills by HPLC[J].Journal of Inner Mongolia University for the Nationalities(Natural Sciences),2009,24(1):82-84. |
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| Authors: | Suritalatu Xi Hai-shan SUN Wurina |
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| Institution: | Suritalatu, Xi Hai-shan, SUN Wurina (1.Affiliated Hospital, Inner Mongolia University for Nationalities ,Tongliao 028000, China; 2.Testing and Analysis Center, Inner Mongolia University for Nationalities, Tongliao 028000,China) |
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| Abstract: | Objective：To find out the optimal detection conditions for the determination of the content of chlorogenic acid in Mongolian medicine Garidi-5 Pills by HPLC.Methods：ODS C18 was taken as chromatographic column.The mobile phase was methyl-water-glacial acetic acid（ll.5： 88.5： 2） with a flow rate of 1.0 ml/min and the wavelength of detection was set at 318nm.Resuits： The linear relation was shown at 4.5-22.5 μg/ml.The average recovery and relative deviation were 98.1% and 2.2% respectively（n=5）.Conclusion： The method is simple,accurate, and can be used to determine the content of chlorogenic acid in Mongolian medicine Garidi-5 Pills. |
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| Keywords: | Garidi-5 Pills HPLC Chlorogenic acid |
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