| 海藻酸钡固定化尿酶电极的研制及应用血清中尿素含量的安培测定 |
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| 引用本文： | 岑传铨.海藻酸钡固定化尿酶电极的研制及应用血清中尿素含量的安培测定[J].华侨大学学报(自然科学版),1991,0(4):440-445. |
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| 作者姓名： | 岑传铨 |
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| 作者单位： | 华侨大学应用化学系 |
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| 基金项目： | 1988年福建省自然科学基金课题. |
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| 摘 要： | 本文报道了用海藻酸钡固定干粉状的尿素酶,并制成酶电极。文中对背景液,选择在 lmmol/L NaH2PO4,lmmol/L Na2HPO4,lmmol/L EDTA,0.1mol/L KCI 10mmol/L 硫酸肼经 NaOH 溶液调节至 pH=7.0的背景液中测定。其电流值与尿素浓度在6×10-5—4×10-3mol/L 间成线性关系,检测限为2×10-5mol/L。本电极以安培法测定血清中尿素氮含量并与尿酶比色法进行比较结果较为满意。经100多次测定电极寿命约10多天,电极的稳定性和重现性良好。
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| 关 键 词： | 尿素 酶电极 固定化酶 血清 尿酶电极 |
An Urease Electrode Prepared by Barium Alginate Immobilizing and Applied to Amperometric Determination of Serum Urea |
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| Cen Chuanquan.An Urease Electrode Prepared by Barium Alginate Immobilizing and Applied to Amperometric Determination of Serum Urea[J].Journal of Huaqiao University(Natural Science),1991,0(4):440-445. |
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| Authors: | Cen Chuanquan |
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| Institution: | Department of Applied Chemistry |
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| Abstract: | An enzyme electrode was prepared by immobilizing the dry powdered urease with barium al- ginate and was applied to the amperometric determination of serum urea nitrogen.The solution contain- ing 1m mol/L NaH2PO4,1m mol/L Na2HPO4,1m mol/L EDTA,0.1mol/L KCl |
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| Keywords: | enzyme electrodes immobilized enzyme urease electrode serum urea nitrogen |
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