醉蝶花的组织培养及其细胞遗传学研究

研究发现赤霉素对醉蝶花(Cleome spinosa Jacq.)种子发芽和幼苗生长有明显的促进作用，其中10mg／L浸种30min的处理对种子发芽和幼苗生长的促进作用最明显，发芽率达到80％．用不同激素组合诱导醉蝶花下胚轴和子叶形成愈伤组织，MS 6-BA0．5mg/L NAA0．5mg/L KT0．5mg／L中子叶的出愈率最高，达到100％．下胚轴出愈不及子叶，最高只有93％．MS 6-BA2mg／L NAA0．05mg/L适合于芽的分化，诱导率达到100％．以醉蝶花幼芽为材料，用常规染色体制片方法确定醉蝶花体细胞染色体数目2n=20．

Studies on Tissue Culture and Cytogenetics of Cleome spinosa Jacq.

Gibberellin can apparently promotes the germination of seeds and the young sprout grow of Cleome spinosa Jacq., it is the best one that the seeds were soaked in 10 mg/L gibberellin aqueous solution for 30 min, the ratio of germination reaches to 80%. Taking MS as basic media, adopting 6-BA,NAA,KT of different concentration to induce callus from Cotyledons and Hypocotyls of Cleome spinosa Jacq., the result showed that the medium of MS 6-BA 0.5mg/L NAA 0.5mg/L KT 0.5mg/L was the best to induce callus from Cotyledons with a ratio of 100%frequency, and the highest ratio of Hypocotyl callus inducement was 93%. And the diffirentiation medium MS 6-BA 2mg/L NAA 0.05mg/L was the best to induce buds and had a 100%plant regeneration. As for rooting media, MS NAA 0.1mg/L can induce roots from seedings. Taking the buds as materials, through normal chromosomes slice methods, it was found that the number of somatic chromosomes of Cleome spinosa Jacq.is 2n=20.