液相色谱测定水样中的氧氟沙星手性对映体

利用大赛路（CHIRALCEL OD-H）手性柱对氧氟沙星对映体进行分离，考察了在流动相组分、流动相添加剂和流速对手性分离的影响。结果表明，V_正己烷∶V_乙醇∶V_乙酸＝80∶20∶0.5的流动相能够使氧氟沙星得到较好的分离，左氧氟沙星和右氧氟沙星的质量浓度在0.25～5.00μg/mL范围内线性关系良好（r²=0.9997）。实际水样中该方法的最低检出限左氧氟沙星为0.28μg/L、右氧氟沙星为0.10μg/L，最低定量限左氧氟沙星为0.36μg/L、右氧氟沙星为0.21μg/L。氧氟沙星的两组浓度加标平均回收率左氧氟沙星90.1%和88.6%、右氧氟沙星为87.6%和85.4%，左氧氟沙星的相对标准偏差（RSD）为5.2%、6.4%，右氧氟沙星的RSD为4.8%、7.6%（n=3）。

Analysis of ofloxacin enantiomers in sewage by high performance liquid chromatography

A comprehensive method has been developed for the analysis of ofloxacin enantiomers in sewage by high performance liquid chromatography coupled with a fluorescence detector. Chiral separation was performed on a CHIRALCEL OD-H column using n-hexane-ethanol-acetic acid as mobile phase at a flow rate of 1.0mL/min. The ofloxacin enantiomers were extracted by a weak cation ion exchange resin (WCX) and eluted with methanol, resulting in ofloxacin recoveries generally above 85%. Good linearities were achieved in the range of 0.25-5.00μg/mL for R-ofloxacin and S-ofloxacin (r²=0.9997). The limits of quantitation (LOQ) were estimated to be about 0.36μg/L for S-ofloxacin and 0-21μg/mL for R-ofloxacin. An actual sample was determined quantitatively using the above method. 