| 不同产地广山楂中金丝桃苷含量比较 |
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| 引用本文： | 覃葆,江海燕,蔡少芳,付晓,邓欢,史勤怡.不同产地广山楂中金丝桃苷含量比较[J].广西科学院学报,2011,27(3):196-198. |
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| 作者姓名： | 覃葆 江海燕 蔡少芳 付晓 邓欢 史勤怡 |
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| 作者单位： | 广西中医学院,广西南宁,530001 |
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| 基金项目： | 广西科技攻关项目(桂科攻0815005-1-9)资助 |
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| 摘 要： | 采用高效液相色谱法测定不同产地的广山楂中金丝桃苷的含量。色谱柱：C18-ODS、流动相：乙腈-0.1%磷酸水溶液（12∶88）、流速：1ml/min、检测波长：360nm、柱温：30℃。结果表明：金丝桃苷在进样量为0.032～1.28μg的范围内呈良好的线性关系（r=0.9999）,平均回收率为98.9%,RSD值为2...
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| 关 键 词： | 广山楂 金丝桃苷 含量比较 |
| 收稿时间： | 2011/5/25 0:00:00 |
Comparison the Content of Hyperoside of Malus Doumeri(Bois) Chev. from Different Producing Area |
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| QIN Bao,JIANG Hai-yan,CAI Shao-fang,FU Xiao,DENG Huan and SHI Qin-yi.Comparison the Content of Hyperoside of Malus Doumeri(Bois) Chev. from Different Producing Area[J].Journal of Guangxi Academy of Sciences,2011,27(3):196-198. |
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| Authors: | QIN Bao JIANG Hai-yan CAI Shao-fang FU Xiao DENG Huan and SHI Qin-yi |
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| Institution: | QIN Bao,JIANG Hai-yan,CAI Shao-fang,FU Xiao,DENG Huan,SHI Qin-yi (Guangxi Traditional Chinese Medical University,Nanning,Guangxi,530001,China) |
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| Abstract: | The content of hyperoside of Malus doumeri（Bois） Chev.from different producing area was determined by HPLC.The C_18 ODS was used at the temperature of 30℃.The mobile phase consisted of acetonitrile and 0.1% orthophosphoric acid（12∶88） with isocratic elution.The UV detector was set at 360nm with 1.0ml/min flow rate.Results showed that a new standard curve,y=1832.3x＋15.938 and r=0.9999,was established.When the hyperoside sample size was between 0.032～1.28μg,the standard curve showed a good linear relationship with 98.9% average recovery and 2.22% RSD.According to the results from HPLC,the content of hyperoside of Malus doumeri（Bois） Chev.was various from different producing area.The highest content of hyperoside was found in Malus doumeri（Bois） Chev.produced in Hezhou. |
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| Keywords: | Malus doumeri(Bois) Chev hyperoside content comparison |
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