Immunocytochemical location of the centrosphere of human cancer cells using natural rabbit antibodies]

Sera of 40 normal nonimmunized rabbits were eprouved by immunoperoxidase on cultural human cells. Four sera contain centrosphere-reactive antibodies and for the strongest serum the centrospheres were still stained with the 1/600 dilution. This last serum visualized the centrospheres within the height human cell lines eprouved, whatever the phase of the mitotic cycle. The staining was not inhibited after serum absorption by tubulin.