SSCP技术在厌氧折流板反应器运行控制中的应用

运用单链构象多态性(SSCP)技术,采用真细菌的通用引物SRV3-2P和BSF8/20,分析了处理大豆蛋白废水的厌氧折流板反应器(ABR)中的厌氧污泥微生物群落结构,并通过UPGMA群落聚类分析方法对各格室细菌的遗传距离进行了分析。结果发现,有机负荷不仅会影响ABR内各细菌类群的生长,同时也会改变细菌群落的结构和不同细菌类群在各格室的分布;随着有机负荷的逐步提高,ABR各格室细菌的遗传距离逐渐增大,特异性随之增加;用SSCP和UPGMA群落聚类分析方法分析污泥样品,可以对ABR的运行起到很好的指导作用。

Analysis of an Anaerobic Baffled Reactor and the Activated Sludge Microorganism Community by Single-strand Conformation Polymorphism

The structure of microorganism community of anaerobic activated sludge from an Anaerobic Baffled Reactor(ABR) for treating soybean wastewater was investigated by Single-Strand Conformation Polymorphism (SSCP) with the eubacterium universal primers SRV3-2P and BSF8/20. The microbial genetic distance among the microorganism community in different compartments was analyzed by UPGMA community clustering method. The results show that the increase of organic loading rate could not only improve the microbe growth in ABR, but also influence the structure and distribution of microbial communities in each compartment. With the increase of the organic loading rate, the genetic distances among the microbial communities in the compartments gradually increase, indicating that the specificity of microbial communities in each compartment is increased. SSCP and UPGMA community clustering of anaerobic activated sludge is very useful in ABR operation.