| He-Ne激光对红曲霉ZL307的诱变育种 |
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| 引用本文： | 张帝,张庆庆,汤文晶,杨超. He-Ne激光对红曲霉ZL307的诱变育种[J]. 安徽工程科技学院学报：自然科学版, 2013, 0(4): 4-7 |
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| 作者姓名： | 张帝 张庆庆 汤文晶 杨超 |
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| 作者单位： | 安徽工程大学生物与化学工程学院; |
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| 基金项目： | 安徽省教育厅自然科学基金资助项目(KJ2009A034);芜湖市重点科技项目(芜科计字[2009]190号文件) |
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| 摘 要： | 以红曲霉ZL307为出发菌株，利用氦-氖（He-Ne）激光对其单孢子悬液进行诱变育种．通过计算存活率和正突变率，确定激光诱变育种的处理条件为电流强度20mA，照射时间50min．通过平板初筛和摇瓶发酵进行复筛，选育出突变株红曲霉ZZ307．相对于出发菌株，实验不仅缩短了菌种的培养时间，而且产色素色价达到100．39U／mL（提高了34％），色价达到峰值时间提前1d．进行10代遗传稳定性实验，突变菌株仍具有良好的遗传稳定性．
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| 关 键 词： | 红曲霉 红曲色素 He-Ne激光 遗传稳定性 |
He-Ne Laser Treatment on Monascus ZL307 |
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| ZHANG Di,ZHANG Qing-qing,TANG Wen-jing,YANG Chao. He-Ne Laser Treatment on Monascus ZL307[J]. Journal of Anhui University of Technology and Science, 2013, 0(4): 4-7 |
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| Authors: | ZHANG Di ZHANG Qing-qing TANG Wen-jing YANG Chao |
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| Affiliation: | (Coll. of Bioch. Engn. ,Anhui Polytechnic University, Wuhu 241000, China) |
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| Abstract: | Monascus ZL307 was used as original strain and the spore suspension underwent He-Ne laser mutation treatment. The optimum conditions：the current intensity was 20 mA and the irradiation time was 50 min were deter mined by calculating the viability and positive mutation rate. Through flat screen- ing and shake flask fermentation rescreening,mutant strain Monascus ZZ307 was obtained,its incubation time of strains was shortened,maximum pigment color value increased by 34% to 100.39 U/mL and the advance time of reaching the peak of color value was 1 d compared with original strain. The heredity characteristics of the mutant strain were stable afte.r subculture for 10 generations. |
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| Keywords: | monascus ~ monascus pigment ~ He-Ne laser ~ heredity stability |
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