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正交设计优化桑黄培养基的研究
引用本文:张革红,刘文艳.正交设计优化桑黄培养基的研究[J].新乡学院学报(自然科学版),2013(4):263-269.
作者姓名:张革红  刘文艳
作者单位:[1]新乡市教育局保健站,河南新乡453000 [2]辉县市国土资源局孟庄国土所,河南辉县453600
摘    要:通过研究不同种类碳源、氮源以及不同浓度的麸皮、葡萄糖、KH2PO4和MgSO4·7H2O对桑黄菌丝生长速度及生长势的影响,采用多因子正交试验确定了培养桑黄的最佳条件.结果表明:在10 g/L葡萄糖、30 g/L麸皮、1.0 g/L KH2PO4、1.0 g/L MgSO4·7H2O,温度26℃和培养时间为12 d的条件下,桑黄菌丝生长速度最大,生长势最好.

关 键 词:桑黄  碳源  氮源  正交试验

Study of Optimizing Phellinus Igniarius Medium by Orthogonal Design
Authors:ZHANG Ge-hong  LIU Wen-yan
Institution:1. Health Station, The Xinxiang City Bureau of Education, Xinxiang 453000, China; 2. Mengzhuang Institute of Land, Huixian Municipal Bureau of Land Resources, Huixian 453600, China)
Abstract:By studying the effects on growth rate and growth potential of different types of carbon and nitrogen sources and different concentrations of wheat bran, glucose, KH2PO4 and MgSO4·7H2O phellinus igniarius mycelium, the best condition for culturing phellinus igniarius is determined by multi-factor orthogonal experiment. Results show that under the conditions of l0 g/L glucose, 30 g/L wheat bran, 1.0 g/L KH2PO4, 1.0 g/L MgSO4·7H20, temperature of 26 ℃ and incubation time of 12 d, phellinus igniarius mycelium has the maximum growth rate and the best growth potential.
Keywords:phellinus igniarius  carbon source  nitrogen source  orthogonal test
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