| Evidence for Base Substitutions and Repair of DNA Mismatch Damage Induced by Low Energy N^＋ Ion Beam Implantation in E. coli |
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| 引用本文： | 谢传晓,Guo Jinhua,Yao Jianmin,Wu Lijun,Yu Zengliang. Evidence for Base Substitutions and Repair of DNA Mismatch Damage Induced by Low Energy N^＋ Ion Beam Implantation in E. coli[J]. 高技术通讯(英文版), 2003, 9(2): 1-6 |
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| 作者姓名： | 谢传晓 Guo Jinhua Yao Jianmin Wu Lijun Yu Zengliang |
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| 作者单位： | [1]KeyLaboratoryofIonBeamBicengineering,ChineseAcademyofSciences.InstituteofPlasmaPhysics,Hefei230031,P.R.China [2]XieChuanxiaoandGuoJinhuaaretheauthorscontributeequallytohispaper,ChineseAcademyofSciences.InstituteofPlasmaPhysics,Hefei230031,P.R.China |
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| 基金项目： | SupportedbyMajorProgramofNationalNaturalScienceFoundation .(No .19890 30 0 ) |
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| 摘 要： | Ever since the low energy N^ ion beam has been accepted, the mutations of ionizing radia-tion are attributable mainly to avoidance of DNA damages repair. Evidences based on in vivo proof results are limited. Using the E. coli wild type and mutator strains, the mutant frequencies suggest that base substitutions in rpoB gene are induced by the N^ implantation. A highly con-served region is selected to get the direct evidence for base substitutions by sequence of the high fi-delity PCR amplification products in mutants. Most of the mutants (90.9%, 40/44) have at least one base substitution in the amplification region. The evidences for CG to TA (55 %, 22/40), AT to GC (20%, 8/40) and TA to CG (5%, 2/40) transitions are identified. The transversions are AT to TA ( 15 %, 6/40) and GC to CG (5 %, 2/40). It is suggested that DNA cytosine methylase might play an important role in mismatch repair of DNA damage induced by N^ implantation by analysis of the mutant frequencies of mutator strains.
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| 关 键 词： | 碱基替换 DNA损害 失配校正 低能N^＋离子束 离子注入 大肠杆菌 诱导效应 |
Evidence for Base Substitutions and Repair of DNA Mismatch Damage Induced by Low Energy N+ Ion Beam Implantation in E. coli |
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| Guo Jinhua,Yao Jianmin,Wu Lijun,Yu Zengliang. Evidence for Base Substitutions and Repair of DNA Mismatch Damage Induced by Low Energy N+ Ion Beam Implantation in E. coli[J]. High Technology Letters, 2003, 9(2): 1-6 |
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| Authors: | Guo Jinhua Yao Jianmin Wu Lijun Yu Zengliang |
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| Affiliation: | Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences.Institute of Plasma Physics, Hefei 230031,P.R.China;Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences.Institute of Plasma Physics, Hefei 230031,P.R.China;Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences.Institute of Plasma Physics, Hefei 230031,P.R.China;Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences.Institute of Plasma Physics, Hefei 230031,P.R.China;Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences.Institute of Plasma Physics, Hefei 230031,P.R.China |
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| Abstract: | Ever since the low energy N + ion beam has been accepted, the mutations of ionizing radiation are attributable mainly to avoidance of DNA damages repair. Evidences based on in vivo proof results are limited. Using the E.coli wild type and mutator strains, the mutant frequencies suggest that base substitutions in rpoB gene are induced by the N + implantation. A highly conserved region is selected to get the direct evidence for base substitutions by sequence of the high fidelity PCR amplification products in mutants. Most of the mutants (90.9%, 40/44) have at least one base substitution in the amplification region. The evidences for CG to TA (55%, 22/40), AT to GC (20%, 8/40) and TA to CG (5%, 2/40) transitions are identified. The transversions are AT to TA (15%, 6/40) and GC to CG (5%, 2/40). It is suggested that DNA cytosine methylase might play an important role in mismatch repair of DNA damage induced by N + implantation by analysis of the mutant frequencies of mutator strains. |
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| Keywords: | low energy N + ion beam base substitutions mismatch repair mutator strains |
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