| BALB／c小鼠选择与腹水产量和抗体效价间关系的研究 |
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| 引用本文： | 杜春红,钟佑宏,陈平,尹家祥,石丽媛,于国林.BALB／c小鼠选择与腹水产量和抗体效价间关系的研究[J].实验动物科学,2008,25(6):9-11. |
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| 作者姓名： | 杜春红 钟佑宏 陈平 尹家祥 石丽媛 于国林 |
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| 作者单位： | 云南省地方病防治所,云南大理,671000 |
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| 摘 要： | 目的观察在鼠疫F1单克隆抗体制备过程中，BALB／c小鼠的选择和饲养与所获腹水量和抗体效价的关系，为大量制备该单克隆抗体提供实验室参考依据。方法根据小鼠周龄、性别和饲养条件对BALB／c小鼠进行分组，并采用动物体内诱生法制备腹水，间接ELISA用来检测腹水中F1单克隆抗体效价。结果12～16周龄组、雄性和加强营养组小鼠所获腹水产量和效价均高于其它周龄组和对照组。结论选择适当周龄的雄性小鼠、饲养过程中加强营养和管理可适当提高腹水产量和抗体的效价。
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| 关 键 词： | F1单克隆抗体 BALB/C小鼠 腹水 |
A Study on Relationship between Selection of BALB/c Mice,Ascitic Yield and Titers of the Antibody |
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| DU Chun-hong,ZHONG You-hong,CHEN Ping,YIN Jia-xiang,SHI Li-yuan,YU Guo-lin.A Study on Relationship between Selection of BALB/c Mice,Ascitic Yield and Titers of the Antibody[J].Shiyan Dongwu Kexue,2008,25(6):9-11. |
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| Authors: | DU Chun-hong ZHONG You-hong CHEN Ping YIN Jia-xiang SHI Li-yuan YU Guo-lin |
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| Institution: | DU Chun-hong,ZHONG You-hong,CHEN Ping,YIN Jia-xiang,SHI Li-yuan,YU Guo-lin(Yunnan Institute for Endemic Disease Control , Prevention,Dali 671000,China) |
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| Abstract: | Objective To observe the relationship between selecting and breeding of BALB/c mice,ascitic yield and titers of the antibodies during the preparation of monoclonal antibodies against Fl antigen of Y.pestis so as to provide reference for preparing vast monoclonal antibodies against Fl antigen of Y.pestis.Methods Ascites was induced in BALB/c mice of different age,sex and breeding conditions,and the titers of monoclonal antibody against Fl antigen of Y.pestis in ascites were detected by indirect ELISA.Results... |
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| Keywords: | Monoclonal antibody against Fl Antigen of Y pestis BALB/c Mice Ascites |
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