| 正交试验法优选坚龙胆中龙胆苦苷的提取工艺 |
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| 引用本文： | 张伟,车安兵,李高明,李娇,王晓芳,肖培云.正交试验法优选坚龙胆中龙胆苦苷的提取工艺[J].大理学院学报,2009,8(6). |
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| 作者姓名： | 张伟 车安兵 李高明 李娇 王晓芳 肖培云 |
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| 作者单位： | 大理学院药学院,云南大理,671000 |
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| 基金项目： | 大理学院大学生科研基金 |
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| 摘 要： | 目的:优选坚龙胆中龙胆苦苷的提取工艺.方法:以龙胆苦苷的含量为指标,采用高效液相色谱法进行含量测定,采用L9(34)正交设计试验,对提取次数、提取溶剂倍数和提取时间3因素进行考察,以确定最佳提取工艺.结果:提取3次,用10倍量的甲醇,提取时间每次20min为最佳提取工艺.结论:该工艺合理,且稳定可行.
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| 关 键 词： | 坚龙胆 龙胆苦苷 正交设计 提取工艺 |
A Study of the Extraction Process for Gentiopicrin in Gentiana Rigescens Franch by Orthogonal Design |
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| Zhang Wei,Che Anbing,Li Gaoming,Li Jiao,Wang Xiaofang,Xiao Peiyun.A Study of the Extraction Process for Gentiopicrin in Gentiana Rigescens Franch by Orthogonal Design[J].Journal of Dali University,2009,8(6). |
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| Authors: | Zhang Wei Che Anbing Li Gaoming Li Jiao Wang Xiaofang Xiao Peiyun |
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| Institution: | Zhang Wei,Che Anbing,Li Gaoming,Li Jiao,Wang Xiaofang,Xiao Peiyun*(College of Pharmacy,Dali University,Dali,Yunnan 671000,China) |
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| Abstract: | Objective: To select the optimum extracting procedure for gentiopicrin from Gentiana rigescens Franch.Methods: The optimum extracting procedure was selected for determination of the content of gentiopicrin from Gentiana rigescens Franch by orthogonal test design.The three factors that may influence the extraction rate: the times of extraction,the solvent concentration times and the extraction time were examined.Results: The optimum extraction condition was 10 times the volume of methanol,if extracted three ... |
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| Keywords: | Gentiana rigescens Franch gentiopicrin orthogonal experimental design extraction process |
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