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| 利用5-氨基乙酰丙酸脱水酶缺失的重组大肠杆菌合成5-氨基乙酰丙酸 | |
| 引用本文： | 郭小飞,陈久洲,张莉露,贾士儒,郑平.利用5-氨基乙酰丙酸脱水酶缺失的重组大肠杆菌合成5-氨基乙酰丙酸[J].天津科技大学学报,2012(4):1-6. |
| 作者姓名： | 郭小飞 陈久洲 张莉露 贾士儒 郑平 |
| 作者单位： | 1. 天津科技大学生物工程学院,天津300457 2. 中国科学院系统微生物工程重点实验室,天津300308 3. 中国科学院天津工业生物技术研究所,天津300308 |
| 基金项目： | 国家自然科学基金资助项目(31070037);中国科学院知识创新工程项目(KSCX2-EW-Q-13) |
| 摘 要： | 生物法合成5-氨基乙酰丙酸(5-ALA)大多通过添加5-ALA脱水酶(ALAD)的抑制剂乙酰丙酸(LA)减少5-ALA的降解,造成生产成本增高,发酵工艺复杂.本文利用ALAD缺失的大肠杆菌ZSEc2作为出发菌株,通过紫外诱变的方法,获得可利用外源血红素恢复正常生长的大肠杆菌突变株ZGEc1,并过表达来自沼泽红假单胞菌的5-ALA合成酶(ALAS)基因,最终建立一条不需要添加ALAD抑制剂的5-ALA的生物合成新路线.经过培养基初步优化,重组菌可在胞外积累约1,g/L的5-ALA. |
| 关 键 词： | 5-氨基乙酰丙酸 5-ALA 脱水酶 5-ALA 合成酶 沼泽红假单胞菌 |
Production of 5-aminolevulinic Acid with 5-aminolevulinic Acid Dehydratase Deficient Escherichia coli Mutant |
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| GUO Xiaofei,CHEN Jiuzhou,ZHANG Lilu,JIAShiru,ZHENG Ping.Production of 5-aminolevulinic Acid with 5-aminolevulinic Acid Dehydratase Deficient Escherichia coli Mutant[J].Journal of Tianjin University of Science & Technology,2012(4):1-6. | |
| Authors: | GUO Xiaofei CHEN Jiuzhou ZHANG Lilu JIAShiru ZHENG Ping |
| Institution: | 2,3(1.College of Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China;2.Key Laboratory of Systems Microbial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China;3.Tianjin Institutes of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China) |
| Abstract: | 5-aminolevulinic acid(5-ALA) is most often biosynthesized by adding 5-ALA dehydratase(ALAD)inhibitorlevulinic acid(LA)to reduce 5-ALA degradation,which leads to the increase of cost and makes the fermentation technology complicated.In this study,we used a 5-ALA dehydratase deficient E.coli mutant ZSEc2,as the starting strain.By applying UV mutagenesis,the mutant ZGEc1,was bred and the strain could retrieve normal growth via consumption of extracellular hemin.Then the 5-ALA synthase from Rhodopseudomonas palustris was over-expressed in the mutant ZGEc1,and a new biosysthesis process of 5-ALA was developed without adding 5-ALA dehydratase inhibiton.After medium optimization,the resultant recombined strain produced about 1,g/L 5-ALA. |
| Keywords: | 5-aminolevulinic acid 5-ALA dehydratase 5-ALA synthase Rhodopseudomonas palustris |
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