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| 绿僵菌发酵液灭杀粘虫活性的研究 | |
| 引用本文： | 柳东,高丙利,朱振,李兴林. 绿僵菌发酵液灭杀粘虫活性的研究[J]. 天津科技大学学报, 2012, 0(2): 9-13,17 |
| 作者姓名： | 柳东 高丙利 朱振 李兴林 |
| 作者单位： | 1. 工业发酵微生物教育部重点实验室,天津科技大学生物工程学院,天津 300457 2. 中国科学院上海生命科学研究院湖州现代农业生物技术产业创新中心,湖州 313000 3. 浙江东奇生物科技有限公司,湖州 313000 |
| 摘 要： | 为了获得有效杀粘虫的生物农药,通过对发酵液杀虫效果的初步测试,从实验室保藏的4株绿僵菌中,筛选出1株效果明显的绿僵菌Ma977.将菌株Ma977的发酵液除去菌体,用其滤液的1、2、4、8倍的稀释液进行室内毒力测试.结果表明:该滤液的原液对粘虫的致死率为100%;随着滤液稀释倍数的增加,致死率逐步降低.对该滤液的有效成分进行液相色谱分析,结果表明,发酵液中含有毒素DtxA、DtxB、DtxB2和DtrxE. |
| 关 键 词： | 绿僵菌 粘虫 发酵液 生物杀虫剂 毒素 |
Insecticidal Activity of Fermentation Broth of Metarhizium anisopliae against Mythimna separata |
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| LIU Dong,GAO Bingli,ZHU Zhen,LI Xinglin. Insecticidal Activity of Fermentation Broth of Metarhizium anisopliae against Mythimna separata[J]. Journal of Tianjin University of Science & Technology, 2012, 0(2): 9-13,17 | |
| Authors: | LIU Dong GAO Bingli ZHU Zhen LI Xinglin |
| Affiliation: | 1(1.Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,College of Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China;2.Huzhou Modern Agricultural Biotechnology Innovation Center,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Huzhou 313000,China;3.Zhejiang Dongqi Biotech Co.,Ltd.,Huzhou 313000,China) |
| Abstract: | In order to obtain an effective bio-pesticide against armyworm,Metarhizium Ma977 that had significant insecticidal activities was selected from 4,Metarhizium strains stored in the laboratory after a primary test of their fermentation broths.After removing Ma977 mycelium and spores,the fermentation broth with 1 time,2 times,4 times and 8 times of dilutions were tested.The results showed that the dilutions from the fermentation broth had an insecticidal effect on armyworm.The effect of the dilutions on armyworm was in relation with their concentrations.The non-diluted broth could kill 100% of the armyworm cultured though the mortality rate decreased along with the consistent dilutions.The effective components in the fermentation broth were further analysized by HPLC.The results indicated that the broth contained 4 destruxins:E,A,B2and B. |
| Keywords: | Metarhizium anisopliae Mythimna separata fermentation broth biopesticide toxin |
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