不同NaCl含量、培养基和微量元素对分枝杆菌SP-3降解菲的影响研究

研究了分枝杆菌SP 3在不同NaCl含量、培养基和微量元素中降解菲的效果影响。结果表明，分枝杆菌SP 3在NaCl质量分数为1%时对菲的降解率达到最大，为87.41%；菌株在LB液体培养基、改良LB液体培养基和改良的无机盐液体培养基中对菲的降解率都达到了80%以上，三者之间无显著性差异；Fe2+、Zn2+浓度分别在100 μmol/L和5 μmol/L，Mg2+在100 μmol/L时，可以提高菌株对菲的降解能力，而Cu2+随着浓度的增加，能够抑制菌株对菲的降解。研究结果有助于该菌应用于多环芳烃降解的生物技术中，以提高菌株对菲等多环芳烃的降解能力。

Impact of different salinity,culture media and trace elements on phenanthrene degradation of Mycobacterium sp. SP-3

We addressed degradation effect of phenanthrene by Mycobacterium sp. SP 3 for different salinity, culture media and trace elements. Results show that degradation rate of phenanthrene is maximum, 87.41%, when salt concentration is 1%. Degradation rate of phenanthrene is more than 80% in LB liquid media, modified LB and modified inorganic salt liquid media, no significant difference among them. Fe2+ concentration of 100 μmol/L, Zn2+ concentration of 5 μmol/L and Mg2+ concentration of 100 μmol/L can increase degradation ability of Mycobacterium sp. SP 3 to phenanthrene. However, Cu2+ can inhibit degradation ability of the strain to phenanthrene with the increase of its concentration. The results will be benefit for the application of the strain to biodegradation of polycyclic aromatic hydrocarbons and the increase of its biodegradation capability to polycyclic aromatic hydrocarbons such as phenanthrene.