| 辣根过氧化物酶在介孔碳CMK-3上在线固定化研究 |
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| 引用本文： | 杨梅,王琳,胡晶,赵叙.辣根过氧化物酶在介孔碳CMK-3上在线固定化研究[J].辽宁师范大学学报(自然科学版),2012(4):508-511. |
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| 作者姓名： | 杨梅 王琳 胡晶 赵叙 |
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| 作者单位： | 辽宁师范大学化学化工学院 |
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| 基金项目： | 国家自然科学基金项目(5151072074) |
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| 摘 要： | 利用顺序注射-紫外可见分光系统,根据介孔材料孔径和生物大分子尺寸相当的特点,采用介孔碳CMK-3为载体实现对辣根过氧化物酶的在线固定化,并实时评价了固定化辣根过氧化物酶的活性及操作稳定性.紫外可见光谱和氮吸附实验结果表明：介孔碳CMK-3能有效吸附辣根过氧化物酶,理论固载量为54.95mg/g;辣根过氧化物酶不仅吸附在CMK-3表面,而且能进入其孔道内,从而增强固定化辣根过氧化物酶的活性稳定性.
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| 关 键 词： | 辣根过氧化酶 介孔碳CMK-3 固定化酶 顺序注射-紫外可见分光系统 |
On-line immobilization of horseradish peroxidase on mesoporous carbon CMK-3 |
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| YANG Mei,WANG Lin,HU Jing,ZHAO Xu.On-line immobilization of horseradish peroxidase on mesoporous carbon CMK-3[J].Journal of Liaoning Normal University(Natural Science Edition),2012(4):508-511. |
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| Authors: | YANG Mei WANG Lin HU Jing ZHAO Xu |
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| Institution: | (School of Chemistry and Chemical Engineering,Liaoning Normal University,Dalian 116029,China) |
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| Abstract: | In the present work,on-line immobilization of horseradish peroxidase（HRP） on mesoporous carbon CMK-3 and real time evaluation of its activity have been researched by employing sequential injection-UV-Vis system.Comparing with the UV-visible spectroscopy of HRP before and after immobilization,it was found that HRP could be efficiently adsorbed by CMK-3 and the adsorption capacity was derived as 54.95 mg/g.The results of the N2 adsorption experiments showed that HRP was adsorbed not only on the surface of CMK-3 but also into the pore of CMK-3,which increased the operational stability of immobilized HRP. |
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| Keywords: | horseradish peroxidase mesoporous carbon CMK-3 immobilized enzyme sequential injection UV-visible spectrometric system |
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