Effects of the adenylate cyclase activator forskolin and its inactive derivative 1,9-dideoxyforskolin on insect cytochrome P-450 dependent steroid hydroxylase activity |
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| Authors: | D. P. Keogh M. J. Mitchell J. R. Crooks S. L. Smith |
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| Affiliation: | (1) Department of Biological Sciences, Bowling Green State University, 43403-0212 Bowling Green, Ohio, USA |
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| Abstract: | The adenylate cyclase activator forskolin and its pharmacologically inactive derivative 1,9-dideoxyforskolin were found to inhibit in a dose-dependent fashion the ecdysone 20-monooxygenase activity associated with wandering stage larvae ofDrosophila melanogaster and fat body and midgut from last instar larvae of the tobacco hornworm,Manduca sexta. The concentrations of these labdane diterpenes required to elicit a 50% inhibition of the cytochrome P-450 dependent steroid hydroxylase activity in the insect tissues ranged from approximately 5×10–6 to 5×10–4 M. |
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| Keywords: | Adenylate cyclase cytochrome P-450 ecdysone 20-monooxygenase forskolin steroid hydroxylase |
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