~1H-NMR法测定饮料中安赛蜜和甜蜜素的含量

采用核磁共振波谱分析技术,对甜味剂安赛蜜和甜蜜素1H-NMR谱图中的各组峰进行了归属。以邻苯二甲酸氢钾做内标,利用安赛蜜和甜蜜素1H-NMR谱图中的主要特征峰,通过标准溶液考察了扫描次数(NS)及弛豫延迟时间(d1)对测定结果的影响。结果表明,安赛蜜和甜蜜素分别在d1=3s和d1=15s时测定结果最佳,NS≥64即可保证基线平直。在最佳条件下对安赛蜜和甜蜜素进行定量测定的线性范围良好,对饮料中的安赛蜜和甜蜜素进行了定量分析,其测定结果的RSD(n=3)分别为0.86%和1.51%,平均回收率分别为96.57%和96.87%。该方法样品处理简单,测定速度快、结果准确,适合于碳酸饮料和茶饮料中安赛蜜和甜蜜素的测定。

Determination of Aciculae potassium and Sodium cyclohexyl sulfamate in drinks by1H- NMR

Characteristic resonance peaks in 1H-NMR spectrum of Acesulfame potassium and Sodium cyclohexyl sulfamate were attributed.The effects of the number of scans(NS) and relaxation delay(d1) on determination of Acesulfame potassium and Sodium cyclohexyl sulfamate were investigated by their characteristic resonance peaks and with Potassium biphthalate as an internal standard.The results showed that d1=3s and d1=15s were the optimum conditions for Acesulfame potassium and Sodium cyclohexyl sulfamate respectively.The baseline can be straight when NS≥64.Under these optimum conditions,linear ranges of Acesulfame potassium and Sodium cyclohexyl sulfamate were excellent.RSD(n=3)of Acesulfame potassium and Sodium cyclohexyl sulfamate were 0.86% and 1.51%,respectively.Average recoveries were 96.57% and 96.87 %,respectively.Sample preparation was simple and rapid,results were accurate.This method was suitable for the determination of Acesulfame potassium and Sodium cyclohexyl sulfamate in carbonated drinks and tea drinks.