Characteristic absorption peak of the human blood measured with differential photoacoustic spectroscopy |
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Authors: | Zhilie Tang Jinlong Liu Yunfei Xia Yong Su Luisheng Liang Gengsheng Chen |
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Institution: | (1) Department of Physics, South China Normal University, 510631 Guangzhou, China;(2) Cancer Center, Sun Yat-Sen University, 510060 Guangzhou, China |
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Abstract: | A new highly sensitive spectroscopy technique- differential photoacoustic spectroscopy (PAS) is presented in this paper. The blood samples from 3 healthy persons, patients with leukemia, patients with pregnancy-induced hypertension (PIH), and 40 patients with nasopharyngeal carcinoma were measured by the PAS technique. The normalized, the first order, and the second order differential photoacoustic spectroscopy of the blood were gained. The results show that (ⅰ) weak absorption peaks or shoulder peaks, which could not be found using conventional photoacoustic spectroscopy, were determined by the first order and the second order differential photoacoustic spectroscopy which significantly improve the sensitivity of detection; and (ii) that two characteristic absorption peaks were found at the wavelength of 637 and 664 nm in all persons' blood samples by the differential photoacoustic spectroscopy technique. This experiment concludes that the differential photoacoustic spectroscopy technique is superior to the conventional photoacoustic spectroscopy technique in detecting photoacoustic spectroscopy of biological samples. |
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Keywords: | photoacoustic spectroscopy differential spectroscopy characteristic absorption peak |
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