| Non-viral gene delivery carrier of probe type host molecule ——Interactions between DNA and β-cyclodextrin derivative complexes (I) |
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| 作者姓名： | LIU Tao CHEN Long HOU Sen XUE Yonglai FENG Xizeng |
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| 作者单位： | Key Laboratory of Bioactive Materials, Ministry of Education, Collegeof Life Science, Nankai University, Tianjin 300071, China |
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| 基金项目： | Acknowledgements The authors would like to thank Dr. and Prof. Lee Im Shik for his generous help in molecular dynamic calculation. This work was supported by the National Natural Science Foundation of China （Grant No. 90403140）, the Tianjin Natural Science Foundation （Grant No. TJ043801111）, and the Scientific Research Foundation for the Returned 0verseas Chinese Scholars, Ministry of Education of China （Grant No. B04970）. |
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| 摘 要： | GENE THERAPY IS A COMMON PROCESS TO DELIVER EXTRIN- SIC GENES INTO TARGET CELLS FOR FURTHER GENE EXPRESSION, FOR THE PURPOSE OF TREATING DISEASES. A WELL DESIGNED GENE DELIVERY CARRIER CAN EFFICIENTLY PACKAGE AND PRO- TECT NUCLEIC ACID FROM BEING DIGESTED BY A VARIETY OF ENZYMES IN VIVO AND SHOULD BE ABLE TO SPECIFICALLY LO- CATE EXTRINSIC GENES INTO THE TARGET ORGANISMS. S…
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| 关 键 词： | phenanthroline-β-cyclodextrin derivatives (DZY-1) λ-DNA non-virus gene delivery carrier agarose gel electrophoresis scanning electron microscope (SEM). |
| 收稿时间： | 2005-08-30 |
| 修稿时间： | 2005-08-302005-10-31 |
Non-viral gene delivery carrier of probe type host molecule—Interactions between DNA and β-cyclodextrin derivative complexes (I) |
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| LIU Tao CHEN Long HOU Sen XUE Yonglai FENG Xizeng.Non-viral gene delivery carrier of probe type host molecule—Interactions between DNA and β-cyclodextrin derivative complexes (I)[J].Chinese Science Bulletin,2006,51(5):530-535. |
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| Authors: | Tao Liu Long Chen Sen Hou Yonglai Xue Xizeng Feng |
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| Institution: | (1) Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, 300071, China |
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| Abstract: | A host type non-virus gene delivery carrier, phenanthroline-β-cyclodextrin derivative host molecule, was produced which can
be used as molecular probe. Interactions between DZY-1 and DNA were investigated by electrophoresis assay. Hind III enzyme inhibition assay was carried out using DNA condensates induced by host molecules or host-guest molecule complexes
to explore their ability to inhibit enzyme digestion. Micro-structure of DNA condensates induced by host molecules and host-guest
molecule complexes was observed by scanning electron microscope (SEM). Our work indicates the delivery mechanism of DZY-1
used as a gene delivery carrier and also provides a method to design and produce non-virus gene delivery carriers. |
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| Keywords: | phenanthroline-β -cyclodextrin derivatives (DZY-1) λ -DNA non-virus gene delivery carrier agarose gel electrophoresis scanning electron microscope (SEM) |
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