| G-8菌株原生质体形成和再生条件的研究 |
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| 引用本文： | 薛林贵,马永智,王维国. G-8菌株原生质体形成和再生条件的研究[J]. 甘肃科学学报, 1999, 11(4): 52-56 |
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| 作者姓名： | 薛林贵 马永智 王维国 |
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| 作者单位： | [1]庆阳师范高等专科学校生物系 [2]庆阳第一中学 |
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| 基金项目： | 甘肃省教委科研基金!（965－08） |
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| 摘 要： | 以浮游球衣细菌Ｇ－８菌为出发菌株，有杉甘氨酸－溶菌酶－ＥＤＴＡ方法，研究了各种因素对原生质体形成和再生的影响。在原生质体形成和再生的最佳条件下制备原生质体，Ｇ－８菌原生质体形成率和一率分别达９６．８％和２９．８％，通过光学显微镜技术，观察了原生质体形成和再生的过程。
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| 关 键 词： | 原生质体 形成 再生 G－8菌株 塑料 生物降解 |
Study on the Condition of Formation and Regeneration of Protoplast of G-8 Strain |
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| XUE Lin-gui,MA Yong-zhi,WANG Wei-guo. Study on the Condition of Formation and Regeneration of Protoplast of G-8 Strain[J]. Journal of Gansu Sciences, 1999, 11(4): 52-56 |
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| Authors: | XUE Lin-gui MA Yong-zhi WANG Wei-guo |
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| Abstract: | All factors were studied.Under the optimum condition of formation and regeneration of protoplasts of primitive strain Sphaerotilus natans G 8 were prepared by glycine|lysozyme|EDTA method .The rates of formation and regeneration of protoplasts of strain G 8 went up to 96.8% and 29.8% respectively .The process of fomation and that of regeneration were observed by optical microscope. |
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| Keywords: | protoplast formation regereration optimum condition |
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