水稻Os05g0442400基因启动子分析以及由Os05g0442400基因启动子引导GUS报告基因在转基因水稻中的表达

采用Plant CARE和PLACE软件分析预测水稻Os05g0442400基因启动子序列中可能存在的顺式作用元件.结果显示,在起始密码子ATG上游1500bp区域内,除了启动子基本的核心作用元件外,还存在一些与植物抵御非生物胁迫过程有关的作用元件、脱落酸应答元件、光诱导启动子作用元件、病原菌诱发因子作用元件,以及根特异性结合位点.采用根癌农杆菌介导法成功将Os05g0442400 promoter::gus构建导入"中花11"水稻.由不同组织部位GUS染液检测表明:在水稻苗期,内源Os05g0442400基因可能主要在根部表达;随着水稻生殖期的延续,水稻内源Os05g0442400基因在颖壳中的表达区域由上向下面积增大,并在抽穗后达到最大表达区域.这些结果可能与Os05g0442400基因启动子上分别存在根特异性结合位点和光诱导启动子作用元件具有一定的联系.

Analysis of the rice Os05g0442400 gene promoter and expression of the GUS fusion gene under control of the rice Os05g0442400 gene promoter in transgenic rice

The promoter sequence of the rice Os05g0442400 gene was analyzed using Plant CARE and PLACE programs. Except the basic promoter c/s - acting elements, several cis - acting elements, including the elements that related to plant resistance to abiotic stress ,light- inducible promoter elements, pathogen- induced factor elements and the root specific motif, were recognized in a 1500bp promoter sequence upstream of the initial codon ATG. The Os05g0442400 promoter： ：gus contruct was transferred in- to rice successfully by Agrobacterium tumefaciens - mediated. The GUS expression in different tissues was assayed using GUS staining. The results showed that endogenous Os05g0442400 gene may mainly expressed in roots. With the continuation of rice re- productive stage, the expression area of Os05g0442400 gene in rice glume increased from top to bottom, and after heading the ex- pression area reached the maximum. These results may have some contact with the root specific motif and light - inducible promot- er elements of Os05g0442400 gene promoter.