| 酚藏花红与DNA作用的光谱研究 |
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| 引用本文： | 高峰,周建庆,王伦,唐莉娟.酚藏花红与DNA作用的光谱研究[J].安徽师范大学学报(自然科学版),2006,29(2):143-147. |
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| 作者姓名： | 高峰 周建庆 王伦 唐莉娟 |
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| 作者单位： | 安徽师范大学,化学与材料科学学院,安徽省功能性分子固体实验室,安徽,芜湖,241000;安徽医学高等专科学校,安徽,合肥,230601 |
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| 基金项目： | 中国科学院资助项目
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NSF of Anhui education committee
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The outstanding teacher project of Anhui Province |
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| 摘 要： | 用光谱法研究了带有吩嗪基团的酚藏花红与DNA的相互作用.研究表明,酚藏花红能嵌入到DNA骨架的碱基对之间,在实验允许的误差范围内,用不同的光谱法测得的结合常数一致,均为104L·mol-1数量级,结合位点数约为7.阴离子KI的荧光猝灭实验进一步证实了酚藏花红与DNA之间的嵌入作用.该研究为以DNA为靶的药物设计提供了一种新的嵌入剂功能团.
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| 关 键 词： | DNA 酚藏花红 嵌入作用 光谱法 |
| 文章编号： | 1001-2443（2006）02-0143-05 |
| 收稿时间： | 2005-05-08 |
| 修稿时间： | 2005年5月8日 |
STUDIES ON THE INTERACTION OF PHENOSAFRANINE WITH DNA BY SPECTROSCOPY |
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| GAO Feng,ZHOU Jian-qing,WANG Lun,TANG Li-juan.STUDIES ON THE INTERACTION OF PHENOSAFRANINE WITH DNA BY SPECTROSCOPY[J].Journal of Anhui Normal University(Natural Science Edition),2006,29(2):143-147. |
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| Authors: | GAO Feng ZHOU Jian-qing WANG Lun TANG Li-juan |
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| Abstract: | The interaction of Phenosafranine(PF), which features a phenazinyl group, with DNA was investigated by spectroscopy. The studies indicated that PF could intercalate into the stack base pairs of DNA. Intrinsic binding constants obtained by different spectral methods were consistent within estimated binding sites are about 7 in DNA base pairs. The results of the studies on fluorescence quenching by anionic quencher KI also confirmed the intercalative binding of PF with DNA. This research proposed a new intercalation functional group to DNA-targeted drug design. |
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| Keywords: | DNA phenosafranine intercalative binding spectroscopy |
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