Loss of antibody production accompanied by chromosome loss in a cloned hybrid line secreting antibodies to sheep red blood cells |
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| Authors: | J S Nowak |
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| Institution: | (1) Institute of Human Genetics Polish Academy of Sciences, PL-60-781 Pozna , Poland |
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| Abstract: | Summary Somatic cell hybrids between Sp2/O-Ag14 mouse myeloma cells and lymphocytes derived from BALB/c mice hyperimmunized with sheep red blood cells (SRBC) were produced. One hybrid producing IgG1 antibody to SRBC was selected, cloned twice and subsequently transferred to BALB/c mice. After a number of transfers it was found that the antibody titer in ascitec fluid gradually decreased. Cytogenetic analysis revealed gradual chromosome loss in the hybrid clone, which produced progressively less antibody. |
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| Keywords: | Myeloma cells mouse lymphocytes mouse cloned hybrid cell line somatic cell hybrids antibody production chromosome loss |
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