| 鹿角菜18S rDNA序列分析及其系统发生分析 |
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| 引用本文： | 刘玮,李美真,詹冬梅,丁刚,吴海一.鹿角菜18S rDNA序列分析及其系统发生分析[J].烟台大学学报(自然科学与工程版),2011,24(1). |
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| 作者姓名： | 刘玮 李美真 詹冬梅 丁刚 吴海一 |
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| 作者单位： | 山东省海水养殖研究所藻类中心实验室,山东,青岛,266002 |
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| 基金项目： | 国家863高技术研究发展计划，山东省科技攻关计划，山东省良种工程重大项目 |
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| 摘 要： | 通过制备鹿角菜DNA,PCR扩增得到鹿角菜18S rDNA序列.测序拼接后全长1733 bp,碱基A、T、C、G含量分别为25.45%、26.72%、26.72%、21.12%,序列已提交Gene Bank登录号为GQ433994.该序列与NCBI数据库中其他褐藻18S rDNA序列比对后,得到可变碱基位点184个,简约信息位点161个,单碱基变化位点23个.转换碱基值Si为44,颠换碱基值Sv为30,转换颠换比值R约为1.5.NJ法构建的系统发生树显示18S rDNA在褐藻门中具有保守性,可用于辅助传统分类.PLACE数据库预测发现在鹿角菜18S rDNA保守区有多个与水分胁迫、光诱导、Ca2+信号传导等相关转录元件,这表明18S rDNA可能参与细胞重要调控途径.
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| 关 键 词： | 鹿角菜 转录因子 系统发生 |
Nucleotide Analysis and Molecular Phylogenetic Affinity of 18S rDNA of Silvetia siliquosa |
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| LIU Wei,LI Mei-zhen,ZHAN Dong-mei,DING Gang,WU Hai-yi.Nucleotide Analysis and Molecular Phylogenetic Affinity of 18S rDNA of Silvetia siliquosa[J].Journal of Yantai University(Natural Science and Engineering edirion),2011,24(1). |
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| Authors: | LIU Wei LI Mei-zhen ZHAN Dong-mei DING Gang WU Hai-yi |
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| Institution: | LIU Wei,LI Mei-zhen,ZHAN Dong-mei,DING Gang,WU Hai-yi(Laboratory of Algal Biotechnology Center,Mariculture Institute of Shandong Province,Qingdao 266002,China) |
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| Abstract: | DNA of Silvetia siliquosa is extracted and the 18S ribosome DNA sequence is amplified using poly chain reaction.The sequence result shows that it is composed of 1733 nucleotides in which the A,T,C and G contents are 25.45%,26.72%,26.72% and 21.12%,respectively.The nucleotides has been submitted to the database of Gene Bank,and accession number is GQ433994.Aligning with other sequences of 18S rDNA in Phaeophyceae,it reveals that there are 184 variable sites,161 parsimonious-information sites and 23 singleton... |
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| Keywords: | 18S rDNA |
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