人类疱疹病毒6、7、8型与巨细胞病毒对大肠肿瘤致病的协同作用

目的:检测HHV-6、7、8及CMV在大肠肿瘤中的感染,探讨其致病的协同作用。方法:取20例大肠肿瘤标本,其中10例良性病变(炎性息肉、乳头状瘤和绒毛状腺瘤),10例恶性病变(癌和腺瘤恶变)。应用巢式PCR法,分别检测两组病变组织中HHV-6、7、8和MCV的DNA片段。结果:HHV-6检测结果表明恶性病变组阳性反应率为90%(9/10),良性病变组阳性反应率为60%(6/10),其产物为132bpDNA片段;HHV-7检测结果示恶性病变组阳性反应率为40%(4/10),良性病变组阳性反应率为0(0/10),其产物为92bpDNA片段;HHV-8检测结果示恶性病变组阳性反应率为90%(9/10),良性病变组阳性反应率为20%(2/10),其产物为177bpDNA片段;检测CMV结果示恶性病变样本组阳性反应率为100%(10/10),良性病变样本组阳性反应率为100%(10/10),其产物为85bpDNA片段。结论:CMV在良、恶性大肠肿瘤病变中均有较高的感染率,HHV-6、7、8在恶性病变组中感染率较良性组高,是需要警惕的致病因素。

Carcinogenic effect of human herpesvirus 6,7,8 and cytomegalovirus in colorectal neoplasm

Aim: To determine the infection of human herpesvirus 6, 7, 8 ( HHV-6, 7, 8 )and cytomegalovirus (CMV) in colorectal neoplasm and discuss their effect on colorectal tumor forming. Methods:Ten cases of malignant neoplasm including colorectal cancer and malignant adenoma and ten cases of benign neoplasm including polypus and papilloma were examined. The DNA segment of HHV-6?7?8 and CMV were checked with nest polymerase chain reaction (PCR) in the cases of two groups.Results:The positive reaction rate of HHV-6?7?8 and CMV in malignant neoplasm group are in turn 9/10?4/10?9/10 and 10/10. In benign neoplasm group the positive reaction are 6/10?0/10?2/10 and 10/10. The size of DNA segment are 132 bp (HHV-6)?92 bp (HHV-7) 177 bp (HHV-8) and 85 bp (CMV).Conclusion:According to the study CMV has a high infection rate in both groups of benign neoplasm and malignant neoplasm, the HHV-6?7?8 have higher infection rate in malignant group than that of in benign group. It should be alerted vigilant that HHV-6?7?8 and CMV are factors for colorectal neoplasm. It is significant to study whether and how the HHV-6?7?8 and CMV relate to colorectal neoplasm.