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| Sm(Ⅲ)-HP配合物与DNA的作用机制 | |
| 引用本文： | 赵娜,王兴明,黎泓波,胡亚敏,费丹,丁立生.Sm(Ⅲ)-HP配合物与DNA的作用机制[J].中山大学学报(自然科学版),2009,48(4). |
| 作者姓名： | 赵娜 王兴明 黎泓波 胡亚敏 费丹 丁立生 |
| 作者单位： | 1. 西南科技大学材料科学与工程学院化学系,四川绵阳,621010 2. 湖南大学化学化工学院,湖南长沙,410082 3. 中国科学院成都生物研究所,四川成都,610041 |
| 基金项目： | 国家自然科学基金资助项目 |
| 摘 要： | 应用紫外-可见光谱法和荧光光谱法并以中性红(NR)作光谱探针研究了Sm(Ⅲ)HP配合物与DNA的作用机制。研究表明，血卟啉(HP)与Sm(Ⅲ)的配合比nSm(Ⅲ)∶nHP = 1∶ 1，Sm(Ⅲ)HP配合物与DNA的结合比nSm(Ⅲ)HP ∶ nDNA = 1∶ 1，Sm(Ⅲ)HP配合物与DNA的结合常数Kθ30℃ = 1.67×104 L/mol。该过程为熵所驱动，其Δ r Hm为4.86×104 J/mol，Δr Sm为195.61 J/(mol·K)，30 ℃时Δr Gm为－1.06×104 J/mol。Scatchard法和探针法研究表明，Sm(Ⅲ)HP与DNA之间的结合方式主要是沟区作用。 |
| 关 键 词： | Sm(Ⅲ)HP配合物 DNA 中性红 作用方式 |
| 收稿时间： | 2008-09-24; |
Functionary Mechanism between Sm (Ⅲ) HP Complex and DNA |
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| ZHAO Na,WANG Xingming,LI Hongbo,HU Yamin,FEI Dan,DING Lisheng.Functionary Mechanism between Sm (Ⅲ) HP Complex and DNA[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2009,48(4). | |
| Authors: | ZHAO Na WANG Xingming LI Hongbo HU Yamin FEI Dan DING Lisheng |
| Institution: | (1. Department of Chemistry, Southwest University of Science and Technology, Mianyang 621010, China;2. Department of Chemistry, Hunan University,Changsha 410082, China;3. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China) |
| Abstract: | Using neutral red (NR) as a probe, functionary mechanism between Sm(Ⅲ)HP and DNA was studied by UV vis and fluorescence spectroscopy. The results indicated that the binding ratio of nSm(Ⅲ) ∶nHP is 1∶1,nSm(Ⅲ)HP ∶nDNA =1∶ 1, and the binding constant of Sm(Ⅲ)HP complex with Herring Sperm DNA was Kθ30℃=1.67×104 L/mol Thermodynamic parameters of enthalpy(Δr Hm), entropy(Δr Sm), and Gibbs free energy(Δr Gm) were calculated to be 4.86×104 J/mol, 195.61 J/(mol·K) and -1.06×104 J/mol, respectively. The results suggested that the process of interaction of Sm(Ⅲ)HP complex and Herring Sperm DNA was driven by entropy. The results of scatchard and probe methods suggested that the primary interaction mode between Sm(Ⅲ) HP and DNA was groove binding. |
| Keywords: | DNA |
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