银杏悬浮培养细胞中黄酮糖苷提取动力学研究

利用醇提法从人工悬浮培养银杏细胞中提取黄酮糖苷。以扩散传质定律为基础，根据质量平衡方程，建立提取过程的动力学模型,结合测得的数据计算出提取过程中的重要动力学参数。结果表明，乙醇法提取银杏细胞黄酮糖苷是从固相到液相的扩散传质过程，属一级反应。建立的动力学模型ρ_FG=ρ_eq｛1-exp［-1.023t·exp(-1469.55/T)］｝能描述银杏细胞黄酮的乙醇法提取过程，高效液相色谱(HPLC)分析表明，人工培养细胞中提取成分主要是以槲皮素、山萘素和异鼠李素与糖结合形成的糖苷。这既促进了生物技术在天然药物生产上的应用，又为黄酮糖苷提取的工艺设计和操作条件选择等方面提供了理论依据。

Kinetics of the extraction of a flavonols glycoside from a suspension of culture cells of Ginkgo biloba

A flavonoid glycoside was extracted with ethanol from an artificial suspension culture at different temperatures. Based on diffusion and mass transfer laws, a kinetic model of flavonoid extraction was established according to the mass balance equation. Key kinetic parameters were calculated based on the data. The results showed that the extraction was a mass transfer process that was first order in flavonoid concentration transferred from the solid phase to the liquid phase. A dynamic model ρ_FG=ρ_eq｛1-exp［-1.023t·exp(-1469.55/T)］｝describes the flavonoid extraction well. HPLC results showed that flavonoid glycoside extracted from artificially cultured cells consisted mainly of quercetin and kaempferol combined sugars. The results not only promote the application of biotechnology in the production of natural medicines, but also provide a theoretical basis for the design and choice of operating conditions of the extraction process.