| 肝质膜蛋白质组研究中染色方法比较及优化 |
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| 引用本文： | 张丽军,魏颖娟,熊继先,贾小芳,谢锦云,梁宋平.肝质膜蛋白质组研究中染色方法比较及优化[J].湖南师范大学自然科学学报,2007,30(1):93-97. |
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| 作者姓名： | 张丽军 魏颖娟 熊继先 贾小芳 谢锦云 梁宋平 |
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| 作者单位： | 湖南师范大学生命科学学院,中国,长沙,410081 |
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| 基金项目： | 国家重大攻关资助项目(2004BA711A11) |
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| 摘 要： | 对肝脏质膜蛋白质组进行了5种染色方法比较实验,发现荧光染的质谱鉴定准确性高于快速银染(Blum银染),快速银染又高于普通银染(Plus OneTM).考染中G-250的效果比R-350好,且背景低、快速.对考染后切取了高丰度蛋白质点后的胶块再进行快速银染,可以既提高鉴定的准确性也不丢失低丰度蛋白质.
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| 关 键 词： | G-250 R-350 银染 荧光染 |
| 文章编号： | 1000-2537（2006）01-0093-05 |
| 收稿时间： | 2006-04-06 |
| 修稿时间： | 04 6 2006 12:00AM |
Compare and Optimization of Staining Methods in Liver Plasma Membrane Proteome Analysis |
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| ZHANG Li-jun,WEI Ying-juan,XIONG Ji-xian,JIA Xiao-fang,XIE Jin-yun,LIANG Song-ping.Compare and Optimization of Staining Methods in Liver Plasma Membrane Proteome Analysis[J].Journal of Natural Science of Hunan Normal University,2007,30(1):93-97. |
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| Authors: | ZHANG Li-jun WEI Ying-juan XIONG Ji-xian JIA Xiao-fang XIE Jin-yun LIANG Song-ping |
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| Institution: | College of Life Science, Hunan Normal University, Changsha 410081, China |
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| Abstract: | Five kinds of staining methods were compared through liver plasma membrane proteomics.With comparing,it was found that the accuracy of Sypro Ruby staining is higher than two silver stainings,of which fast silver staining(Blum silver staining)is better than general silver staining(PlusOneTM silver staining).The effect of G-250 staining is better than that of R-350,with quick speed and low background.Fast silver staining following coomassie blue staining can not only improve the accuracy of protein identification,but also identify more low-abundance proteins. |
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| Keywords: | G-250 R-350 silver staining Sypro Ruby staining |
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