| 果实采后病害拮抗菌的筛选及鉴定 |
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| 引用本文： | 宋聪,李正国,边万平,杨迎伍,王中康.果实采后病害拮抗菌的筛选及鉴定[J].西南师范大学学报(自然科学版),2007,32(2):76-81. |
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| 作者姓名： | 宋聪 李正国 边万平 杨迎伍 王中康 |
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| 作者单位： | 重庆大学,生物工程学院基因工程研究中心,重庆,400044;重庆市高校功能基因及调控技术重点实验室,重庆,400044;重庆市杀虫真菌农药工程技术研究中心,重庆,40044 |
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| 基金项目： | 重庆市科委自然科学基金项目（8479）,重庆市科委攻关项目（038001）资助. |
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| 摘 要： | 从水果、叶片表面及果园土壤中分离到76株细菌和26株酵母菌，通过平板对峙法和果实接种法筛选到1株细菌2株酵母菌，对梨黑斑病菌（Alternaria kikuchiana）、柑橘青霉病菌（Penicillium italicum）都表现出显著的拮抗效果．根据其形态特征和生理生化特性以及细菌的16SrDNA序列的进化树分析，初步鉴定这3株菌分别为蜡样芽孢杆菌（Bacillus cereus），假丝酵母（Candida sp．）和克勒克酵母（Kloeckea sp．）．
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| 关 键 词： | 果实 采后病害 拮抗菌 生物防治 |
| 文章编号： | 1000-5471（2007）02-0076-06 |
| 修稿时间： | 2006-11-24 |
Isolation and Selection of Antagonists for Fruit Postharvest Diseases |
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| SONG Cong,LI Zheng-guo,BIAN Wan-ping,YANG Ying-wu,Wang Zhong-kang.Isolation and Selection of Antagonists for Fruit Postharvest Diseases[J].Journal of Southwest China Normal University(Natural Science),2007,32(2):76-81. |
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| Authors: | SONG Cong LI Zheng-guo BIAN Wan-ping YANG Ying-wu Wang Zhong-kang |
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| Institution: | 1.Bio-Engineering College, Genetic Engineering Research Center, Chongqing University, Chongqing 400044, China ;2. Key Lab of Functional Gene and Regulation Technologies under Chongqing Municipal Education Commission, Chonging 400044, China ;3.Engineering and Technology Research Center for Fungal Insecticides of Chongqing , Chongqing 400044, China |
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| Abstract: | 76 bacteria and 26 yeasts strains were isolated from the surface of fruits, leaves and orchard soil, and one bacterium and two yeasts were selected through in vitro and in vivo methods. All of them showed significant activities to inhibit some pathogenic fungi of postharvest diseases such as Alternaria kikuchiana and Penicillium italicum. They have been identified as Bacillus cereus, Candida sp. and Kloeckea sp. , according to their morphological, physiological, biochemical characters and the analysis of the sequence of 16S rDNA. |
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| Keywords: | fruit postharvest diseases antagonists biocontol |
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