| 纤维素酶益生菌的选育及产酶条件 |
| |
| 引用本文： | 叶明,叶崇军,胡士明,刘惠东,贾光蕾,王凤美.纤维素酶益生菌的选育及产酶条件[J].合肥工业大学学报(自然科学版),2009,32(11). |
| |
| 作者姓名： | 叶明 叶崇军 胡士明 刘惠东 贾光蕾 王凤美 |
| |
| 作者单位： | 1. 合肥工业大学,生物与食品工程学院,安徽,合肥,230009
2. 安徽九牛集团,安徽,合肥,230031 |
| |
| 基金项目： | 安徽省重点科研计划资助项目，安徽省企业技术创新资助项目 |
| |
| 摘 要： | 从土壤中筛选出一株产纤维素酶的细菌B1,通过形态观察、生理生化特征及16S rDNA同源性序列分析,鉴定为蜡样芽孢杆菌(Bacillus cereus).经微波-硫酸二乙酯复合诱变筛选出正突变株B1-1,其纤维素酶酶活达到0.373 U/mL,经5次传代后,该菌株遗传性状稳定,且胃肠道耐受性良好;B1-1菌株最佳产酶条件为20 g/L麦芽糖,2.5 g/L硫酸铵,pH值为5.0,37 ℃下培养36 h.
|
| 关 键 词： | 纤维素酶 复合诱变 产酶条件 益生菌 |
Reseach on induced breeding and production condition of cellulose-producing probiotics |
| |
| YE Ming,YE Chong-jun,HU Shi-ming,LIU Hui-dong,JIA Guang-lei,WANG Feng-mei.Reseach on induced breeding and production condition of cellulose-producing probiotics[J].Journal of Hefei University of Technology(Natural Science),2009,32(11). |
| |
| Authors: | YE Ming YE Chong-jun HU Shi-ming LIU Hui-dong JIA Guang-lei WANG Feng-mei |
| |
| Abstract: | Strain Bl with cellulase activity was screened from soil, which was identified as Bacillus cereus according to the characteristics of morphology, physiology and biochemistry tests and the comparison of 16S rD-NA sequence. Strain Bl-1 with high cellulase activity was filtrated by microwave and DES mutagenesis, and its cellulase activity reached 0. 373 U/mL. Strain Bl-1 was genetically stable after five subcultures and well survived in the gastrointestinal tract The optimum conditions for cellulase production were 20 g/L maltose, 2. 5 g/L ammonium sulphate, pH 5. 0 and culturing for 36 h at 37℃. |
| |
| Keywords: | 16SrDNA |
| 本文献已被 万方数据 等数据库收录！ |
|
