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| Inducing dopaminergic differentiation of expanded rat mesencephalic neural stem cells by ascorbic acid in vitro | |
| 作者姓名： | ZHENG Min WANG Dongmei HOU Lingling LI Haimin XIE Chao JIAO Wencang BAI Cixian WANG Yaping PEI Xuetao |
| 作者单位： | Department of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China;Institute of Basic Medical Sciences, Chongqing University of Medical Sciences, Chongqing 400016, China,Department of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China,Department of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China,Department of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China,Department of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China,Department of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China,Department of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China,Institute of Basic Medical Sciences, Chongqing University of Medical Sciences, Chongqing 400016, China,Department of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China |
| 基金项目： | 国家重点基础研究发展计划(973计划)，国家高技术研究发展计划(863计划) |
| 摘 要： | Ascorbic acid (AA) induced differentiation of neural stem cells (NSCs) into dopaminergic (DAergic) neurons is reported.NSCs derived from rat mesencephalon were maintained and expanded in a defined medium containing mitogens of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF).Compared with the control, ascorbic acid treatment led to more DAergic neuronal differentiation as indicated by the expression of tyrosine hydroxylase (TH) and dopamine transporter (DAT), which are specific markers of dopamine neurons.AA induction also enhanced expression of Nurr1 and Shh.PD98059, an inhibitor of mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) pathway, could block AA-induced Nurr1, TH and DAT mRNA expression.The results might suggest a new strategy to provide enough dopaminergic cells for the therapy of Parkinson's disease (PD), and Nurr1 and ERK signaling pathway might participate in the AA-induced DAergic differentiation. |
| 关 键 词： | neural stem cells differentiation Parkinson's disease mitogen-activated protein kinase(MAPK)/extr |
Inducing dopaminergic differentiation of expanded rat mesencephalic neural stem cells by ascorbic acid in vitro |
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| ZHENG Min,WANG Dongmei,HOU Lingling,LI Haimin,XIE Chao,JIAO Wencang,BAI Cixian,WANG Yaping,PEI Xuetao.Inducing dopaminergic differentiation of expanded rat mesencephalic neural stem cells by ascorbic acid in vitro[J].Progress in Natural Science,2004,14(1):26-30. | |
| Authors: | ZHENG Min WANG Dongmei HOU Lingling LI Haimin Xie Chao JIAO Wencang BAI Cixian WANG Yaping Pei Xuetao |
| Institution: | 1. Department of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China;Institute of Basic Medical Sciences, Chongqing University of Medical Sciences, Chongqing 400016, China 2. Department of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China 3. Institute of Basic Medical Sciences, Chongqing University of Medical Sciences, Chongqing 400016, China |
| Abstract: | Ascorbic acid (AA) induced differentiation of neural stem cells (NSCs) into dopaminergic (DAergic) neurons is reported.NSCs derived from rat mesencephalon were maintained and expanded in a defined medium containing mitogens of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF).Compared with the control, ascorbic acid treatment led to more DAergic neuronal differentiation as indicated by the expression of tyrosine hydroxylase (TH) and dopamine transporter (DAT), which are specific markers of dopamine neurons.AA induction also enhanced expression of Nurr1 and Shh.PD98059, an inhibitor of mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) pathway, could block AA-induced Nurr1, TH and DAT mRNA expression.The results might suggest a new strategy to provide enough dopaminergic cells for the therapy of Parkinson's disease (PD), and Nurr1 and ERK signaling pathway might participate in the AA-induced DAergic differentiation. |
| Keywords: | neural stem cells differentiation Parkinson's disease mitogen-activated protein kinase(MAPK)/extracellular signal-regulated kinase (ERK) pathway signal transduction |
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