| H2S对ox-HDL致内皮细胞损伤的保护作用 |
| |
| 引用本文： | 彭湘萍,彭振宇.H2S对ox-HDL致内皮细胞损伤的保护作用[J].吉首大学学报(自然科学版),2011,32(2):108-110. |
| |
| 作者姓名： | 彭湘萍 彭振宇 |
| |
| 作者单位： | （1.吉首大学医学院，湖南 吉首 416000；2.吉首大学第一附属医院，湖南 吉首 416000) |
| |
| 基金项目： | 吉首大学校级科研项目资助(09JD019) |
| |
| 摘 要： | 探讨硫化氢对氧化高密度脂蛋白（ox-HDL）致人脐静脉内皮细胞（HUVECs）损伤的保护作用.方法 体外培养HUVECs细胞，应用形态学观察法和噻唑兰（MTT）比色法检测细胞活性，试剂盒测定培养液中乳酸脱氢酶(LDH) 及丙二醛(MDA)含量.结果 ox-HDL组细胞损伤明显,经硫化氢低、中、高剂量组的干预，受损情况均有较明显改善,细胞存活率增加，且培养液中LDH及MDA含量降低，与对照组比较差异有显著性(P<0.05).结论 硫化氢对ox-HDL所致内皮细胞损伤具有一定的保护作用，其机制可能与降低氧自由基抗氧化作用有关.
|
| 关 键 词： | 硫化氢 氧化高密度脂蛋白 内皮细胞 损伤 保护 |
Protective Effect of Hydrogen Sulfide on the Injury of Endothelial Cell Induced by Oxidized HDL |
| |
| PENG Xiang-ping,PENG Zhen-yu.Protective Effect of Hydrogen Sulfide on the Injury of Endothelial Cell Induced by Oxidized HDL[J].Journal of Jishou University(Natural Science Edition),2011,32(2):108-110. |
| |
| Authors: | PENG Xiang-ping PENG Zhen-yu |
| |
| Institution: | (1.Medical College,Jishou University,Jishou 416000,Hunan China;2.First Affiliated Hospital of Jishou University,Jishou 416000,Hunan China) |
| |
| Abstract: | Objective To investigate the protective effect of hydrogen sulfide on the injury of endothelial cell induced by ox-HDL.Methods HUVECs were incubated in vitro.The cells viability is measured by morphological observation and the MTT assay.The levels of LDH and MDA activity in culture medium were measured with corresponding kit.Results HUVECs were damaged obviously in ox-HDL group,hydrogen sulfide improved the condition of HUVEC significantly,increased the cell survival rate,decreased the level of LDH and MDA(... |
| |
| Keywords: | hydrogen sulfide ox-HDL endothelial cell injury protective |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《吉首大学学报(自然科学版)》浏览原始摘要信息 |
| 点击此处可从《吉首大学学报(自然科学版)》下载免费的PDF全文 |
