| Fine mapping of the dominant glandless Gene Gl2^e in Sea-island cotton （Gossypium barbadense L.） |
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| 作者姓名： | DONG ChengGuang DING YeZhang GUO WangZhen ZHANG TianZhen |
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| 作者单位： | National Key Laboratory of Crop Genetics ＆ Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, China |
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| 基金项目： | Supported by the National Basic Research Program of China (Grant No. 2007CB108805)，Program for Changjiang Scholars and Innovative Re-search Team in University (Grant No. IRT0432) |
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| 摘 要： | Gle2 is a mutant gene that controls glandless trait in cotton plants and seeds. It is an important gene resource to gossypol-free cottonseed breeding. The objective of this research was to develop SSR markers tightly linked with Gle2 by using the F2 segregating population containing 1599 plants derived from the cross of G. hirsutum genetic standard line TM-1 and G. barbadense glandless mutant line Hai-1. Genetic analysis suggested that the Gle2 was an incomplete dominant gene. Based on the backbone of genetic linkage map from G. hirsutum × G. barbadense BC1 published by our laboratory,Gle2 was lo-cated between CIR362 and NAU2251b,NAU3860b,STV033,with a genetic distance 9.27 and 0.96 cM,respectively. This result is useful for cloning Gle2 gene by map-based cloning method.
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| 关 键 词： | 显性 突变基因 棉花 种子 |
| 收稿时间： | 18 June 2007 |
| 修稿时间： | 2007-06-18 |
Fine mapping of the dominant glandless Gene Gle2 in Sea-island cotton (Gossypium barbadense L.) |
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| DONG ChengGuang DING YeZhang GUO WangZhen ZHANG TianZhen.Fine mapping of the dominant glandless Gene Gle2 in Sea-island cotton (Gossypium barbadense L.)[J].Chinese Science Bulletin,2007,52(22):3105-3109. |
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| Authors: | DONG ChengGuang DING YeZhang GUO WangZhen & ZHANG TianZhen |
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| Institution: | (1) National Key Laboratory of Crop Genetics & Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing, 210095, China |
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| Abstract: | Gl2^e is a mutant gene that controls glandless trait in cotton plants and seeds. It is an important gene resource to gossypol-free cottonseed breeding. The objective of this research was to develop SSR markers tightly linked with Gl2^e by using the F2 segregating population containing 1599 plants derived from the cross of G hirsutum genetic standard line TM-1 and G barbadense glandless mutant line Hai-1. Genetic analysis suggested that the Gl2^e was an incomplete dominant gene. Based on the backbone of genetic linkage map from G. hirsutum x G. barbadense BC1 published by our laboratory, Gl2^e was Iocared between CIR362 and NAU2251b, NAU3860b, STV033, with a genetic distance 9.27 and 0.96 cM, respectively. This result is useful for cloning Gl2^e gene by map-based cloning method. |
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| Keywords: | cotton Gl2e gene glandless frait molecular marker fine mapping |
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