| Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR |
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| 作者姓名： | MI LiJuan ZHU HongPing ZHANG XiaoDong HU Jun FAN ChunHai |
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| 作者单位： | [1]Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China [2]Bio-X Life Research center, College of Life Science and Biotechnology, ShanghaiJiao TongUn＇iversity, Shanghai 200030, China; [3]Graduate University of Chinese Academy of Sciences, Beijing 100049 China |
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| 基金项目： | Supported by the National Natural Science Foundation of China （Grant Nos. 10335070, 60537030, and 20404016）, the Major State Research Development Pro- gram of China （Grant No. 2006CB933000）, Shanghai Municipal Commission for Science and Technology （Grant No. 0652NM006） and the Chinese Academy of Sci- |
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| 摘 要： | Nanoparticle PCR is a novel method to optimize DNA amplification. It performs well in improving specificity, enhancing sensitivity and speed. Several mechanisms were proposed in previous studies: one was based on the interaction between gold nanoparticles (AuNPs) and DNA while the other was attributed to the heat transfer property of AuNPs. In this paper, we propose that the interaction between AuNPs and DNA polymerase can significantly influence PCR. First, the addition of DNA polymerase can eliminate the inhibitory effects of excess AuNPs. Second, the addition of AuNPs will increase yield of the desired PCR product and make the optimum concentration of DNA polymerase move to higher value. Third, while excess polymerase might inhibit amplification efficiency, AuNPs can reverse this process and the yield of PCR amplification. Based on these results we propose a possible mechanism that AuNPs might modulate the activity of polymerase and improve PCR amplification.
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| 关 键 词： | 纳米科技 金纳米颗粒 聚合酶 反应机制 |
| 收稿时间： | 17 November 2006 |
| 修稿时间： | 2006-11-17 |
Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR |
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| MI LiJuan ZHU HongPing ZHANG XiaoDong HU Jun FAN ChunHai.Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR[J].Chinese Science Bulletin,2007,52(17):2345-2349. |
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| Authors: | Mi LiJuan Zhu HongPing Zhang XiaoDong Hu Jun Fan ChunHai |
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| Institution: | (1) Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, China;(2) Bio-X Life Research Center, College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200030, China;(3) Graduate University of Chinese Academy of Sciences, Beijing, 100049, China |
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| Abstract: | Nanoparticle PCR is a novel method to optimize DNA amplification. It performs well in improving specificity, enhancing sensitivity and speed. Several mechanisms were proposed in previous studies: one was based on the interaction between gold nanoparticles (AuNPs) and DNA while the other was attributed to the heat transfer property of AuNPs. In this paper, we propose that the interaction between AuNPs and DNA polymerase can significantly influence PCR. First, the addition of DNA polymerase can eliminate the inhibitory effects of excess AuNPs. Second, the addition of AuNPs will increase yield of the desired PCR product and make the optimum concentration of DNA polymerase move to higher value. Third, while excess polymerase might inhibit amplification efficiency, AuNPs can reverse this process and the yield of PCR amplification. Based on these results we propose a possible mechanism that AuNPs might modulate the activity of polymerase and improve PCR amplification. |
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| Keywords: | PCR nanoparticles Au/gold nanoparticle polymerase mechanism |
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